Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein
文献类型：期刊论文
| 作者 | Li, GC; Li, N; Zhang, YH; Li, X; Wang, YG; Liu, XY; Qian, WB; Liu, XC |
| 刊名 | INTERNATIONAL JOURNAL OF HEMATOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 89期号:5页码:611-617 |
| 关键词 | Mannose Myeloid leukemia Pinellia pedatisecta agglutinin Adenovirus |
| 通讯作者 | Qian, WB (reprint author), Zhejiang Univ, Coll Med, Dept Hematol, Affiliated Hosp 1, Hangzhou 310003, Zhejiang, Peoples R China.,qianwenb@yahoo.com.cn ; xcliu@zstu.edu.cn |
| 英文摘要 | Altered glycosylation may be a hallmark of malignant transformation and cancer progression. In the work described, a specific mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was genetically fused with the extracellular domain of coxsackie-adenovirus receptor (CAR) to generate the soluble CAR (sCAR)-PPA fusion protein. The adenoviral transduction of acute myeloid leukemia (AML) cell lines Kasumi-1 and HL-60 was increased by sCAR-PPA, indicating that a fraction of AML cells exposing mannose residues was detected by PPA. However, sCAR-PPA did not increase the adenoviral infection of KG-1 cells, suggesting the mannose exposure of AML cells may be cell type specific. Furthermore, the infectious efficiency of Ad-EGFP in chronic myeloid leukemia cell line K562 was significantly increased by sCAR-PPA as well. We, herein, report that PPA recognized a fraction of myeloid leukemia cells showing mannose-exposing phenotype. The sCAR-PPA fusion protein combined with the adenoviral vector system may provide a useful tool for investigating myeloid leukemia cells exposing mannose residues and further elucidating the role of these cells in the leukemia development. |
| 学科主题 | Hematology |
| 类目[WOS] | Hematology |
| 关键词[WOS] | ACUTE LYMPHOBLASTIC-LEUKEMIA ; N-ACETYLGLUCOSAMINYLTRANSFERASE-V ; COLON-CANCER CELLS ; BINDING LECTIN ; PINELLIA-PEDATISECTA ; ADENOVIRUS VECTOR ; EXPRESSION ; CHILDHOOD ; ADHESION ; GLYCANS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000266583200008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1190]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, GC,Li, N,Zhang, YH,et al. Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein[J]. INTERNATIONAL JOURNAL OF HEMATOLOGY,2009,89(5):611-617. |
| APA | Li, GC.,Li, N.,Zhang, YH.,Li, X.,Wang, YG.,...&Liu, XC.(2009).Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein.INTERNATIONAL JOURNAL OF HEMATOLOGY,89(5),611-617. |
| MLA | Li, GC,et al."Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein".INTERNATIONAL JOURNAL OF HEMATOLOGY 89.5(2009):611-617. |
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