Modification of hepatitis C virus 1b RNA polymerase to make a highly active JFH1-type polymerase by mutation of the thumb domain
文献类型：期刊论文
| 作者 | Weng, LY; Du, JM; Zhou, JL; Ding, JP; Wakita, T; Kohara, M; Toyoda, T |
| 刊名 | ARCHIVES OF VIROLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 154期号:5页码:765-773 |
| 通讯作者 | Toyoda, T (reprint author), Chinese Acad Prevent Med, Unit Viral Genome Regulat, Inst Pasteur Shanghai, 225 S Chongqing Rd, Shanghai 200025, Peoples R China.,ttoyoda@sibs.ac.cn |
| 英文摘要 | Hepatitis C virus (HCV) JFH1 efficiently replicates and produces infectious virus particles in cultured cells. We compared polymerase activity between JFH1 and 1b strains in vitro. The RNA polymerase activity of 1b was 6.4% of that of JFH1. In order to study the mechanism and identify domains responsible for the high polymerase activity of JFH1, we converted the amino acids of 1b RdRp to those of JFH1, and compared their Km, Vmax and template binding activity. Four amino acid mutations in the thumb domain of 1b RdRp, S377R, A450S, E455N and Y561F increased 1b polymerase activity, and their activity was 23.1, 45.8, 28.9, and 36.1% of JFH1, respectively. Vmax and RNA binding activity of JFH1, 1bwt and 1bA450S was JFH1 > 1bA450S > 1b, which indicated both high processivity and slightly higher template binding activity contributed to the high polymerase activity of JFH1. |
| 学科主题 | Virology |
| 类目[WOS] | Virology |
| 关键词[WOS] | PROTEIN 5B POLYMERASE ; DE-NOVO INITIATION ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; NS5B ; GENOME ; IDENTIFICATION ; REQUIREMENTS ; REPLICATION ; MECHANISM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265915300004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1192]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Weng, LY,Du, JM,Zhou, JL,et al. Modification of hepatitis C virus 1b RNA polymerase to make a highly active JFH1-type polymerase by mutation of the thumb domain[J]. ARCHIVES OF VIROLOGY,2009,154(5):765-773. |
| APA | Weng, LY.,Du, JM.,Zhou, JL.,Ding, JP.,Wakita, T.,...&Toyoda, T.(2009).Modification of hepatitis C virus 1b RNA polymerase to make a highly active JFH1-type polymerase by mutation of the thumb domain.ARCHIVES OF VIROLOGY,154(5),765-773. |
| MLA | Weng, LY,et al."Modification of hepatitis C virus 1b RNA polymerase to make a highly active JFH1-type polymerase by mutation of the thumb domain".ARCHIVES OF VIROLOGY 154.5(2009):765-773. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。