Temporal and spatial profiling of nuclei-associated proteins upon TNF-alpha/NF-kappa B signaling
文献类型：期刊论文
| 作者 | Ma, DJ; Li, SJ; Wang, LS; Dai, J; Zhao, SL; Zeng, R |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 19期号:5页码:651-664 |
| 关键词 | quantitative analysis SILAC proteomics TNF-alpha/NF-kappa B nucleus FANCD2 subcellular fractionation |
| 通讯作者 | Zeng, R (reprint author), Chinese Acad Sci, Key Lab Syst Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn |
| 英文摘要 | The tumor necrosis factor (TNF)-alpha/NF-kappa B-signaling pathway plays a pivotal role in various processes including apoptosis, cellular differentiation, host defense, inflammation, autoimmunity and organogenesis. The complexity of the TNF-alpha/NF-kappa B signaling is in part due to the dynamic protein behaviors of key players in this pathway. In this present work, a dynamic and global view of the signaling components in the nucleus at the early stages of TNF-alpha/NF-kappa B signaling was obtained in HEK293 cells, by a combination of subcellular fractionation and stable isotope labeling by amino acids in cell culture (SILAC). The dynamic profile patterns of 547 TNF-alpha-induced nuclei-associated proteins were quantified in our studies. The functional characters of all the profiles were further analyzed using that Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation. Additionally, many previously unknown effectors of TNF-alpha/NF-kappa B signaling were identified, quantified and clustered into differential activation profiles. Interestingly, levels of Fanconi anemia group D2 protein (FANCD2), one of the Fanconi anemia family proteins, was found to be increased in the nucleus by SILAC quantitation upon TNF-alpha stimulation, which was further verified by western blotting and immunofluorescence analysis. This indicates that FANCD2 might be involved in TNF-alpha/NF-kappa B signaling through its accumulation in the nucleus. In summary, the combination of subcellular proteomics with quantitative analysis not only allowed for a dissection of the nuclear TNF-alpha/NF-kappa B-signaling pathway, but also provided a systematic strategy for monitoring temporal and spatial changes in cell signaling. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | MASS-SPECTROMETRY ; FACTOR RECEPTOR-1 ; GENE-EXPRESSION ; TFII-I ; ACTIVATION ; PATHWAY ; PROTEOMICS ; GROWTH ; PHOSPHORYLATION ; CHROMATOGRAPHY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000266527600013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1193]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ma, DJ,Li, SJ,Wang, LS,et al. Temporal and spatial profiling of nuclei-associated proteins upon TNF-alpha/NF-kappa B signaling[J]. CELL RESEARCH,2009,19(5):651-664. |
| APA | Ma, DJ,Li, SJ,Wang, LS,Dai, J,Zhao, SL,&Zeng, R.(2009).Temporal and spatial profiling of nuclei-associated proteins upon TNF-alpha/NF-kappa B signaling.CELL RESEARCH,19(5),651-664. |
| MLA | Ma, DJ,et al."Temporal and spatial profiling of nuclei-associated proteins upon TNF-alpha/NF-kappa B signaling".CELL RESEARCH 19.5(2009):651-664. |
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