In vitro and in vivo evaluation of small interference RNA-mediated gynaecophoral canal protein silencing in Schistosoma japonicum
文献类型：期刊论文
| 作者 | Cheng, GF; Fu, ZQ; Lin, JJ; Shi, Y; Zhou, YC; Jin, YX; Cai, YM |
| 刊名 | JOURNAL OF GENE MEDICINE
 |
| 出版日期 | 2009 |
| 卷号 | 11期号:5页码:412-421 |
| 关键词 | gynaecophoral canal protein pairing RNA interference Schistosoma japonicum schistosomiasis |
| 通讯作者 | Cai, YM (reprint author), 518 Zi Yue Rd, Shanghai 200241, Peoples R China.,cheng_guofeng@yahoo.com ; yxjin@sibs.ac.cn |
| 英文摘要 | Background Schistosomiasis causes liver and intestinal damage and can be very debilitating. The pairing of a male worm with a female worm residing in the gynaecophoral canal of male plays a critical role in the development of female parasite. Because the male specific gynaecophoral canal protein of Schistosoma japonicum (SjGCP) is found in significant quantities in the adult female worm after pairing, it could play an important role in parasite pairing. Methods in the present study, three small interfering (si)RNA duplexes targeting the SjGCP gene were designed, synthesized and the silencing effects were evaluated in vitro as well as in mice infected with S. japonicum in vivo. Results In vitro studies using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time RT-PCR revealed the reduction of SjGCP at the transcript level. Similarly, western blotting and immunofluorescence studies showed its reduction at the protein level after treatment of parasites with siRNAs. At a concentration of 200 nm, two siRNAs totally abolished the parasite pairing. To evaluate such a pairing inhibitory effect in vivo, mice infected with S. japonicum were treated with siRNA and both parasite pairing and burden were evaluated. In vivo tests confirmed the in vitro silencing effect of SjGCP siRNA and revealed that the systemic delivery of siRNA significantly inhibited early parasite pairing and the associated burden. Conclusions Our preliminary results demonstrated that the SjGCP plays an important role in pairing and subsequent development in S. japonicum, and its silencing might have potential as a therapeutic approach for controlling schistosomiasis. Copyright (C) 2009 John Wiley & Sons, Ltd. |
| 学科主题 | Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental |
| 关键词[WOS] | MALE WORMS ; REPRODUCTIVE DEVELOPMENT ; GENE-EXPRESSION ; EGG-PRODUCTION ; FEMALE WORMS ; MANSONI ; MALES ; GLYCOPROTEIN ; STIMULATION ; CHOLESTEROL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265985700005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1195]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Cheng, GF,Fu, ZQ,Lin, JJ,et al. In vitro and in vivo evaluation of small interference RNA-mediated gynaecophoral canal protein silencing in Schistosoma japonicum[J]. JOURNAL OF GENE MEDICINE,2009,11(5):412-421. |
| APA | Cheng, GF.,Fu, ZQ.,Lin, JJ.,Shi, Y.,Zhou, YC.,...&Cai, YM.(2009).In vitro and in vivo evaluation of small interference RNA-mediated gynaecophoral canal protein silencing in Schistosoma japonicum.JOURNAL OF GENE MEDICINE,11(5),412-421. |
| MLA | Cheng, GF,et al."In vitro and in vivo evaluation of small interference RNA-mediated gynaecophoral canal protein silencing in Schistosoma japonicum".JOURNAL OF GENE MEDICINE 11.5(2009):412-421. |
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