Generation and Application of Active Recombinant Mouse Tissue Factor and Its Function-blocking Monoclonal Antibody
文献类型：期刊论文
| 作者 | Pei, XH; Chi, S; Wang, JT; Lin, ZX; Zhang, XH; Geng, JG |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2009 |
| 卷号 | 36期号:5页码:601-607 |
| 关键词 | mouse tissue factor baculovirus expression system Sf9 cells function-blocking antibody |
| 通讯作者 | Geng, JG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,genglab@gmail.com |
| 英文摘要 | Tissue factor (TF) is a cell surface glycoprotein playing an important role in the initiation of the blood coagulation cascade. The functions of TF in other physiological or pathological activities, such as tumor cell migration, are also acknowledged gradually in recent years. A recombinant protein of mouse tissue factor (mTF) extracellular part fused with His tag was constructed, and it was expressed and purified successfully in high-level soluble form. This recombinant mTF can effectively initiate plasma clotting in vitro and enhance blood coagulation in vivo, which prove its biological activity. Using this recombinant mTF as immunogen, a murine monoclonal function-blocking antibody to mTF was also generated, which can inhibit mTF-initiated plasma clotting in vitro. And by treating with this function-blocking antibody, the thrombus formation in a murine deep vein thrombosis model was attenuated successfully, which suggests the important role of tissue factor in deep vein thrombosis. In all, with the active mTF recombinant protein and the mTF function-blocking antibody, the functional investigations of TF in murine models of various research areas become more convenient and feasible. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ENDOTHELIAL-CELLS ; FACTOR EXPRESSION ; FACTOR GENE ; COAGULATION ; HEMOSTASIS ; THROMBIN ; EVENTS |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000266394600012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1198]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Pei, XH,Chi, S,Wang, JT,et al. Generation and Application of Active Recombinant Mouse Tissue Factor and Its Function-blocking Monoclonal Antibody[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2009,36(5):601-607. |
| APA | Pei, XH,Chi, S,Wang, JT,Lin, ZX,Zhang, XH,&Geng, JG.(2009).Generation and Application of Active Recombinant Mouse Tissue Factor and Its Function-blocking Monoclonal Antibody.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,36(5),601-607. |
| MLA | Pei, XH,et al."Generation and Application of Active Recombinant Mouse Tissue Factor and Its Function-blocking Monoclonal Antibody".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 36.5(2009):601-607. |
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