Structural modeling and biochemical studies reveal insights into the molecular basis of the recognition of beta-2-microglobulin by antibody BBM.1
文献类型：期刊论文
| 作者 | Du, JM; Yang, H; Peng, BZ; Ding, JP |
| 刊名 | JOURNAL OF MOLECULAR RECOGNITION
 |
| 出版日期 | 2009 |
| 卷号 | 22期号:6页码:465-473 |
| 关键词 | antibody-antigen complex beta 2m BBM.1 docking HLA-1 |
| 通讯作者 | Ding, JP (reprint author), Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,jpding@sibs.ac.cn |
| 英文摘要 | Human beta-2-microglobulin (beta 2m) is the light chain of human leucocyte antigen-I (HLA-1). It can disassociate from HLA-I and accumulate to cause serious dialysis-related amyloidosis (DRA) in long-term hemodialysis patients. Monoclonal antibody (mAb) BBM.1 can recognize both free-form and HLA-I associated beta 2m. It can be used for specific elimination of beta 2m from serum and can induce apoptosis of several types of tumor cells, and thus has great therapeutic potential. In this study, we constructed structural models of the BBM.1 Fv (fragment of the variable domain) and the BBM.1 Fv-beta 2m complex, followed by biochemical evaluation. Analysis of the optimal complex model reveals that the previously identified immunodominant residues Glu(44) and Arg(45) of beta 2m have direct interactions with BBM.1, while Asp(38) exerts its function mainly via stabilization of Arg(45). In addition, Arg(81) of beta 2m is a newly identified immunodominant residue to have direct interaction with BBM.1. Further modeling study shows no steric conflict between the antibody and the HLA-I heavy chain. These results provide insights into the molecular basis of the recognition of beta 2m by BBM.1 and explain why BBM.1 can bind both free-form and HLA-1 associated beta 2m. This information could be exploited in the engineering and improvement of BBM.1 and the development of other beta 2m-targeting mAbs for therapeutic purposes. Copyright (C) 2009 John Wiley & Sons, Ltd. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | MONOCLONAL-ANTIBODIES ; SERUM BETA-2-MICROGLOBULIN ; RHEUMATOID-ARTHRITIS ; CLASS-I ; PROTEIN ; BETA(2)-MICROGLOBULIN ; AMYLOIDOSIS ; APOPTOSIS ; ANTIGENS ; DETERMINANTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000271342800006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1202]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Du, JM,Yang, H,Peng, BZ,et al. Structural modeling and biochemical studies reveal insights into the molecular basis of the recognition of beta-2-microglobulin by antibody BBM.1[J]. JOURNAL OF MOLECULAR RECOGNITION,2009,22(6):465-473. |
| APA | Du, JM,Yang, H,Peng, BZ,&Ding, JP.(2009).Structural modeling and biochemical studies reveal insights into the molecular basis of the recognition of beta-2-microglobulin by antibody BBM.1.JOURNAL OF MOLECULAR RECOGNITION,22(6),465-473. |
| MLA | Du, JM,et al."Structural modeling and biochemical studies reveal insights into the molecular basis of the recognition of beta-2-microglobulin by antibody BBM.1".JOURNAL OF MOLECULAR RECOGNITION 22.6(2009):465-473. |
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