Utilization of an intron located polyadenlyation site resulted in four novel glutamate decarboxylase transcripts
文献类型：期刊论文
| 作者 | Liu, HX; Wang, ZQ; Li, SF; Zhang, YB; Yan, YC; Li, YP |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2009 |
| 卷号 | 36期号:6页码:1469-1474 |
| 关键词 | GAD Splicing Polyadenylation Post-transcription |
| 通讯作者 | Li, YP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab,Shanghai Key Lab Mol Androl, Yue Yang Rd, Shanghai 200031, Peoples R China.,Yipingli@sibs.ac.cn |
| 英文摘要 | Glutamate decarboxylase (GAD) is the rate-limiting enzyme in the synthesis of gamma-aminobutyric acid (GABA), the most important inhibitory neurotransmitter in central nervous system (CNS). Two homologous forms of GAD encoded by separate genes have been identified in mammalian brain, with molecular weight of 65 kDa (GAD65) and 67 kDa (GAD67). In the present study, four novel GAD67 transcripts produced by alternative splicing and polyadenlyation were cloned from rat testis. These novel GAD67 transcripts were widely expressed in non-neuronal tissues. During rat testis maturation, their expression level showed a time dependent change. These transcripts were predicted to synthesis of GAD proteins truncated of the binding site for pyridoxal phosphate, an essential cofactor, therefore cannot function as a decarboxylase. Thus, post-transcriptional processing mechanism as alternative splicing and polyadenlyation may play a crucial role in regulating rat GAD67 gene expression. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ACID DECARBOXYLASE ; MESSENGER-RNAS ; POLY(A) SITE ; ALTERNATIVE POLYADENYLATION ; EXPRESSION ; PROTEIN ; GENE ; SELECTION ; FORMS ; UNITS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000266454500036 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1204]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, HX,Wang, ZQ,Li, SF,et al. Utilization of an intron located polyadenlyation site resulted in four novel glutamate decarboxylase transcripts[J]. MOLECULAR BIOLOGY REPORTS,2009,36(6):1469-1474. |
| APA | Liu, HX,Wang, ZQ,Li, SF,Zhang, YB,Yan, YC,&Li, YP.(2009).Utilization of an intron located polyadenlyation site resulted in four novel glutamate decarboxylase transcripts.MOLECULAR BIOLOGY REPORTS,36(6),1469-1474. |
| MLA | Liu, HX,et al."Utilization of an intron located polyadenlyation site resulted in four novel glutamate decarboxylase transcripts".MOLECULAR BIOLOGY REPORTS 36.6(2009):1469-1474. |
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