CIN85 associates with endosomal membrane and binds phosphatidic acid
文献类型：期刊论文
| 作者 | Zhang, J; Zheng, XD; Yang, X; Liao, K |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 19期号:6页码:733-746 |
| 关键词 | CIN85 the coiled-coil domain phosphatidic acid EGFR endocytosis ESCRT assembly |
| 通讯作者 | Liao, K (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn |
| 英文摘要 | CIN85 (Cbl-interacting protein of 85 kDa) is an important molecule involved in receptor tyrosine kinase endocytosis. Here we report that through its positively charged C-terminus, CIN85 associates with a fusogenic lipid - phosphatidic acid. Its coiled-coil domain plays an important role in mediating this protein-lipid interaction. Deletion of the coiled-coil domain results in loss of membrane association, and reduced interaction with c-cbl, finally causing the blockage of epidermal growth factor receptor downregulation. In addition, a significant portion of CIN85 is located on the endosomal compartment and is related to endocytic cargo sorting, characterized by CIN85's localization on the "E class" compartment and EGF degradation blockage in CIN85 knockdown cells. Taken together, our results suggest that CIN85 may function as a scaffold molecule in both the internalization and endocytic cargo sorting processes through its association with the endosomal membrane. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | RECEPTOR DOWN-REGULATION ; GROWTH-FACTOR RECEPTORS ; PHOSPHOLIPASE-D ; TYROSINE KINASES ; MAMMALIAN-CELLS ; EGF RECEPTORS ; C-CBL ; PROTEIN ; COMPLEX ; TRAFFICKING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000266528000007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1207]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, J,Zheng, XD,Yang, X,et al. CIN85 associates with endosomal membrane and binds phosphatidic acid[J]. CELL RESEARCH,2009,19(6):733-746. |
| APA | Zhang, J,Zheng, XD,Yang, X,&Liao, K.(2009).CIN85 associates with endosomal membrane and binds phosphatidic acid.CELL RESEARCH,19(6),733-746. |
| MLA | Zhang, J,et al."CIN85 associates with endosomal membrane and binds phosphatidic acid".CELL RESEARCH 19.6(2009):733-746. |
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