Auto- and Cross-Regulation of the hnRNP L Proteins by Alternative Splicing
文献类型：期刊论文
| 作者 | Rossbach, O; Hung, LH; Schreiner, S; Grishina, I; Heiner, M; Hui, JY; Bindereif, A |
| 刊名 | MOLECULAR AND CELLULAR BIOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 29期号:6页码:1442-1451 |
| 通讯作者 | Bindereif, A (reprint author), Univ Giessen, Inst Biochem, Heinrich Buff Ring 58, D-35392 Giessen, Germany.,albrecht.bindereif@chemie.bio.uni-giessen.de |
| 英文摘要 | We recently characterized human hnRNP L as a global regulator of alternative splicing, binding to CA-repeat and CA-rich elements. Here we report that hnRNP L autoregulates its own expression on the level of alternative splicing. Intron 6 of the human hnRNP L gene contains a short exon that, if used, introduces a premature termination codon, resulting in nonsense-mediated decay (NMD). This "poison exon" is preceded by a highly conserved CA-rich cluster extending over 800 nucleotides that binds hnRNP L and functions as an unusually extended, intronic enhancer, promoting inclusion of the poison exon. As a result, excess hnRNP L activates NMD of its own mRNA, thereby creating a negative autoregulatory feedback loop and contributing to homeostasis of hnRNP L levels. We present experimental evidence for this mechanism, based on NMD inactivation, hnRNP L binding assays, and hnRNP L-dependent alternative splicing of heterologous constructs. In addition, we demonstrate that hnRNP L cross-regulates inclusion of an analogous poison exon in the hnRNP L-like pre-mRNA, which explains the reciprocal expression of the two closely related hnRNP L proteins. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | MESSENGER-RNA DECAY ; NONSENSE-MEDIATED DECAY ; GENE-EXPRESSION ; CA REPEATS ; NEURONAL DIFFERENTIATION ; COMBINATORIAL CONTROL ; EXON ; ELEMENTS ; BINDING ; RIBONUCLEOPROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000263720000006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1213]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Rossbach, O,Hung, LH,Schreiner, S,et al. Auto- and Cross-Regulation of the hnRNP L Proteins by Alternative Splicing[J]. MOLECULAR AND CELLULAR BIOLOGY,2009,29(6):1442-1451. |
| APA | Rossbach, O.,Hung, LH.,Schreiner, S.,Grishina, I.,Heiner, M.,...&Bindereif, A.(2009).Auto- and Cross-Regulation of the hnRNP L Proteins by Alternative Splicing.MOLECULAR AND CELLULAR BIOLOGY,29(6),1442-1451. |
| MLA | Rossbach, O,et al."Auto- and Cross-Regulation of the hnRNP L Proteins by Alternative Splicing".MOLECULAR AND CELLULAR BIOLOGY 29.6(2009):1442-1451. |
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