Dynamic regulation of glutamic acid decarboxylase 65 gene expression in rat testis
文献类型：期刊论文
| 作者 | Liu, HX; Li, SF; Zhang, YB; Yan, YC; Li, YP |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2009 |
| 卷号 | 41期号:7页码:545-553 |
| 关键词 | transcription gene regulation glutamate decarboxylase testis |
| 通讯作者 | Li, YP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci,Grad Sch, Shanghai Key Lab Mol Androl, Lab Mol Cell Biol,Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,yipingli@sibs.ac.cn |
| 英文摘要 | Glutamate decarboxylase 65 (GAD65) produces gamma-aminobutyric acid, the main inhibitory neurotransmitter in adult mammalian brain. Previous experiments, performed in brain, showed that GAD65 gene possesses two TATA-less promoters, although the significance is unknown. Here, by rapid amplification of cDNA ends method, two distinct GAD65 mRNA isoforms transcribed from two independent clusters of transcription start sites were identified in post-natal rat testis. RT-PCR results revealed that the two mRNA isoforms had distinct expression patterns during post-natal testis maturation, suggesting that GAD65 gene expression was regulated by alternative promoters at the transcription level. By using GAD65-specific antibodies, western blotting analysis showed that the 58-kDa GAD65, N-terminal 69 amino acids truncated form of full-length GAD65 protein, was developmentally expressed during post-natal testis maturation, suggesting that GAD65 gene expression in testis may also be regulated by post-translational processing. Confocal immunofluorescence microscopy revealed that GAD65 protein was presented in Leydig cells of Day 1 testis, primary spermatocytes and spermatids of postnatal of Day 90 testis. The above results suggested that GAD65 gene expression is dynamically regulated at multiple levels during post-natal testis maturation. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PANCREATIC BETA-CELLS ; MALE GERM-CELLS ; SPERMATOGENIC CELLS ; GABA(A) RECEPTORS ; NEURONS ; IDENTIFICATION ; MOUSE ; BRAIN ; PROMOTER ; STORAGE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268889000003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1217]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, HX,Li, SF,Zhang, YB,et al. Dynamic regulation of glutamic acid decarboxylase 65 gene expression in rat testis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(7):545-553. |
| APA | Liu, HX,Li, SF,Zhang, YB,Yan, YC,&Li, YP.(2009).Dynamic regulation of glutamic acid decarboxylase 65 gene expression in rat testis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(7),545-553. |
| MLA | Liu, HX,et al."Dynamic regulation of glutamic acid decarboxylase 65 gene expression in rat testis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.7(2009):545-553. |
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