Novel function of perforin in negatively regulating CD4(+) T cell activation by affecting calcium signaling
文献类型：期刊论文
| 作者 | Bi, EG; Huang, CJ; Hu, Y; Wu, XD; Deng, WW; Lin, GM; Liu, ZD; Tian, L; Sun, SH; Mao, KR |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 19期号:7页码:816-827 |
| 关键词 | perforin T cell activation TCR signal autoimmune disease Ca2+ signaling |
| 通讯作者 | Sun, B (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,bsun@sibs.ac.cn |
| 英文摘要 | Perforin is a pore-forming protein engaged mainly in mediating target T cell death and is employed by cytotoxic T lymphocytes (CTLs) and natural killer cells. However, whether it also plays a role in conventional CD4(+) T cell function remains unclear. Here we report that in perforin-deficient (PKO) mice, CD4(+) T cells are hyperproliferative in response to T cell receptor (TCR) stimulation. This feature of hyperproliferation is accompanied by the enhancement both in cell division and in IL-2 secretion. It seems that the perforin deficiency does not influence T cell development in thymus spleen and lymph node. In vivo, perforin deficiency results in increased antigen-specific T cell proliferation and antibody production. Furthermore, PKO mice are more susceptible to experimental autoimmune uveitis. To address the molecular mechanism, we found that after TCR stimulation, CD4(+) T cells from PKO mice display an increased intracellular calcium flux and subsequently enhance activation of transcription factor NFAT1. Our results indicate that perforin plays a negative role in regulating CD4(+) T cell activation and immune response by affecting TCR-dependent Ca2+ signaling. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | ANTIGEN-RECEPTOR ; DENDRITIC CELLS ; DEFICIENT MICE ; IFN-GAMMA ; CALRETICULIN ; PROTEIN ; STORES ; GENE ; OVEREXPRESSION ; DEPLETION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268722200004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1219]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Bi, EG,Huang, CJ,Hu, Y,et al. Novel function of perforin in negatively regulating CD4(+) T cell activation by affecting calcium signaling[J]. CELL RESEARCH,2009,19(7):816-827. |
| APA | Bi, EG.,Huang, CJ.,Hu, Y.,Wu, XD.,Deng, WW.,...&Sun, B.(2009).Novel function of perforin in negatively regulating CD4(+) T cell activation by affecting calcium signaling.CELL RESEARCH,19(7),816-827. |
| MLA | Bi, EG,et al."Novel function of perforin in negatively regulating CD4(+) T cell activation by affecting calcium signaling".CELL RESEARCH 19.7(2009):816-827. |
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