Ten1p promotes the telomeric DNA-binding activity of Cdc13p: implication for its function in telomere length regulation
文献类型：期刊论文
| 作者 | Qian, W; Wang, JY; Jin, NN; Fu, XH; Lin, YC; Lin, JJ; Zhou, JQ |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 19期号:7页码:849-863 |
| 关键词 | telomere Cdc13 Ten1 Stn1 |
| 通讯作者 | Zhou, JQ (reprint author), Chinese Acad Sci, Grad Sch, Inst Biochem & Cell Biol, State Key Lab Mol Biol,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,jqzhou@sibs.ac.cn |
| 英文摘要 | In Saccharomyces cerevisiae, the essential gene CDC13 encodes a telomeric single-stranded DNA-binding protein that interacts with Stn1p and Ten1p genetically and physically, and is required for telomere end protection and telomere length control. The molecular mechanism by which Ten1 participates in telomere length regulation and chromosome end protection remains elusive. In this work, we observed a weak interaction of Cdc13p and Ten1p in a gel-filtration analysis using purified recombinant Cdc13p and Ten1p. Ten1p itself exhibits a weak DNA-binding activity, but enhances the telomeric TG(1-3) DNA-binding ability of Cdc13p. Cdc13p is co-immunoprecipitated with Ten1p. In the mutant ten1-55 or ten1-66 cells, the impaired interaction between Ten1p and Cdc13p results in much longer telomeres, as well as a decreased association of Cdc13p with telomeric DNA. Consistently, the Ten1-55 and Ten1-66 mutant proteins fail to stimulate the telomeric DNA-binding activity of Cdc13p in vitro. These results suggest that Ten1p enhances the telomeric DNA-binding activity of Cdc13p to negatively regulate telomere length. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | SINGLE-STRANDED-DNA ; SACCHAROMYCES-CEREVISIAE ; CATALYTIC SUBUNIT ; POLYMERASE-ALPHA ; END PROTECTION ; IN-VITRO ; YEAST ; PROTEIN ; STN1 ; REPLICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268722200007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1220]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Qian, W,Wang, JY,Jin, NN,et al. Ten1p promotes the telomeric DNA-binding activity of Cdc13p: implication for its function in telomere length regulation[J]. CELL RESEARCH,2009,19(7):849-863. |
| APA | Qian, W.,Wang, JY.,Jin, NN.,Fu, XH.,Lin, YC.,...&Zhou, JQ.(2009).Ten1p promotes the telomeric DNA-binding activity of Cdc13p: implication for its function in telomere length regulation.CELL RESEARCH,19(7),849-863. |
| MLA | Qian, W,et al."Ten1p promotes the telomeric DNA-binding activity of Cdc13p: implication for its function in telomere length regulation".CELL RESEARCH 19.7(2009):849-863. |
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