Expression and function on embryonic development of lissencephaly-1 genes in zebrafish
文献类型：期刊论文
| 作者 | Sun, CF; Xu, MF; Xing, Z; Wu, ZL; Li, YP; Li, TP; Zhao, MJ |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2009 |
| 卷号 | 41期号:8页码:677-688 |
| 关键词 | LIS1 expression embryonic development zebrafish |
| 通讯作者 | Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,mjzhao@sibs.ac.cn |
| 英文摘要 | Lissencephaly is a severe disease characterized by brain malformation. The main causative gene of lissencephaly is LIS1. Mutation or deletion of LIS1 leads to proliferation and migration deficiency of neurons in brain development. However, little is known about its biological function in embryonic development. In this article, we identified the expression patterns of zebrafish LIS1 gene and investigated its function in embryonic development. We demonstrated that zebrafish consisted of two LIS1 genes, LIS1a and LIS1b. Bioinformatics analysis revealed that LIS1 genes were conserved in evolution both in protein sequences and genomic structures. The expression patterns of zebrafish LIS1a and LIS1b showed that both transcripts were ubiquitously expressed at all embryonic developmental stages and in adult tissues examined. At the protein level, the LIS1 products mainly exist in brain tissue and in embryos at early stages as shown by western blotting analysis. The whole-mount immunostaining data showed that LIS1 proteins were distributed all over the embryos from 1-cell stage to 5 day post-fertilization. Knockdown of LIS1 protein expression through morpholino antisense oligonucleotides resulted in many developmental deficiencies in zebrafish, including brain malformation, circulation abnormality, and body curl. Taken together, our study suggested that zebrafish LIS1 plays a very important role in embryonic development. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PLATELET-ACTIVATING-FACTOR ; NEURONAL MIGRATION ; NUCLEAR MIGRATION ; CELL-DIVISION ; LIS1 ; PROTEIN ; SUBUNIT ; DYNEIN ; DIFFERENTIATION ; REDUCTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268807900007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1223]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Sun, CF,Xu, MF,Xing, Z,et al. Expression and function on embryonic development of lissencephaly-1 genes in zebrafish[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(8):677-688. |
| APA | Sun, CF.,Xu, MF.,Xing, Z.,Wu, ZL.,Li, YP.,...&Zhao, MJ.(2009).Expression and function on embryonic development of lissencephaly-1 genes in zebrafish.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(8),677-688. |
| MLA | Sun, CF,et al."Expression and function on embryonic development of lissencephaly-1 genes in zebrafish".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.8(2009):677-688. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。