Evidences of Hfq associates with tryptophanase and affects extracellular indole levels
文献类型：期刊论文
| 作者 | Zhang, YH; Hong, GF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2009 |
| 卷号 | 41期号:8页码:709-717 |
| 关键词 | Hfq tryptophanase indole |
| 通讯作者 | Hong, GF (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,gfhong@sibs.ac.cn |
| 英文摘要 | In this study, we observed a novel property of Escherichia coli Hfq protein: it possibly influenced extracellular indole levels. The extracellular indole concentrations were increased in Hfq mutant cells and decreased in Hfq overexpression cells in a cell density-dependent manner. The decreased extracellular indole levels in Hfq overexpression cells caused the postponement of entering into stationary phase. Indole was produced by tryptophanase, the gene product of tnaA, which catalyzed tryptophan into indole, ammonia and pyruvate. Further studies showed that at cell density of 0.8 but not at 0.4, tryptophanase activities of total cell extracts were affected by Hfq mutation or overexpression. Protein pull-down assay and co-immunoprecipitation experiments revealed that Hfq associated with tryptophanase under relatively higher extracellular indole levels, suggesting this was a feedback control of indole production. The association of Hfq and tryptophanase might be indirect because purified Hfq could not affect the values of K-m and V-max of purified tryptophanase. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ESCHERICHIA-COLI K-12 ; ACID RESISTANCE ; REGULATORY ROLE ; Q-BETA ; RNA ; GENES ; PROTEIN ; IDENTIFICATION ; PSEUDOMONAS ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268807900010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1224]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, YH,Hong, GF. Evidences of Hfq associates with tryptophanase and affects extracellular indole levels[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(8):709-717. |
| APA | Zhang, YH,&Hong, GF.(2009).Evidences of Hfq associates with tryptophanase and affects extracellular indole levels.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(8),709-717. |
| MLA | Zhang, YH,et al."Evidences of Hfq associates with tryptophanase and affects extracellular indole levels".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.8(2009):709-717. |
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