NDST1-dependent heparan sulfate regulates BMP signaling and internalization in lung development
文献类型：期刊论文
| 作者 | Hu, ZH; Wang, CC; Xiao, Y; Sheng, NY; Chen, YB; Xu, Y; Zhang, L; Mo, W; Jing, NH; Hu, GX |
| 刊名 | JOURNAL OF CELL SCIENCE
 |
| 出版日期 | 2009 |
| 卷号 | 122期号:8页码:1145-1154 |
| 关键词 | NDST1 BMP signaling Lung development |
| 通讯作者 | Hu, GX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,hgxgene@sunm.shcnc.ac.cn |
| 英文摘要 | Heparan sulfate proteoglycans (HSPGs) are required for various signaling pathways, one of which is the bone morphogenetic protein (BMP) signaling pathway. N-deacetylase/N-sulfotransferase-1 (NDST1) participates in synthesizing heparan sulfate (HS) chains of HSPGs, and is involved in bone and lung development. Here, we report that in spite of the redundant expression of Ndst2, Ndst3 and Ndst4 genes, Ndst1(-/-) mice display defective differentiation of lung cells and increased cell proliferation. Loss of Ndst1 in the lung enhances downstream BMP signaling in vivo. Noggin, which is an antagonist of BMP, can rescue the Ndst1(-/-) lung morphogenetic defects in explant cultures. Further studies in vitro indicated that loss of Ndst1 significantly impairs BMP internalization by decreasing BMP binding to endogenous HS. Exogenous heparin can rescue both the BMP signaling and BMP internalization abnormalities in Ndst1(-/-) lung. Thus, we propose that HS regulates BMP signaling by controlling the balance between BMP binding to HS, and that BMP receptors and NDST1-dependent modification are essential for this process. The results suggest that NDST1-dependent HS is essential for proper functioning of BMP in embryonic lung development. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | N-DEACETYLASE/N-SULFOTRANSFERASE ; MOUSE EMBRYONIC LUNG ; BONE MORPHOGENETIC PROTEIN-4 ; BRANCHING-MORPHOGENESIS ; MOLECULAR-CLONING ; SPEMANN ORGANIZER ; GENE-EXPRESSION ; LACRIMAL-GLAND ; MAST-CELLS ; PROTEOGLYCANS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264777700011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1231]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, ZH,Wang, CC,Xiao, Y,et al. NDST1-dependent heparan sulfate regulates BMP signaling and internalization in lung development[J]. JOURNAL OF CELL SCIENCE,2009,122(8):1145-1154. |
| APA | Hu, ZH.,Wang, CC.,Xiao, Y.,Sheng, NY.,Chen, YB.,...&Hu, GX.(2009).NDST1-dependent heparan sulfate regulates BMP signaling and internalization in lung development.JOURNAL OF CELL SCIENCE,122(8),1145-1154. |
| MLA | Hu, ZH,et al."NDST1-dependent heparan sulfate regulates BMP signaling and internalization in lung development".JOURNAL OF CELL SCIENCE 122.8(2009):1145-1154. |
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