Post-transcriptional regulation of NifA expression by Hfq and RNase E complex in Rhizobium leguminosarum bv. viciae
文献类型：期刊论文
| 作者 | Zhang, YH; Hong, GF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2009 |
| 卷号 | 41期号:9页码:719-730 |
| 关键词 | Hfq RNase E NifA Rhizobium leguminosarum bv viciae |
| 通讯作者 | Hong, GF (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,gfhong@sibs.ac.cn |
| 英文摘要 | NifA is the general transcriptional activator of nitrogen fixation genes in diazotrophic bacteria. In Rhizobium leguminosarum bv. viciae strain 8401/pRL1JI, the NifA gene is part of a gene cluster (fixABCXNifAB). In this study, results showed that in R. leguminosarum bv. viciae 8401/pRL1JI, host factor required (Hfq), and RNase E were involved in the post-transcriptional regulation of NifA expression. It was found that Hfq-dependent RNase E cleavage of NifA mRNA was essential for NifA translation. The cleavage site is located at 32 nucleotides upstream of the NifA translational start codon. A possible explanation based on predicted RNA secondary structure of the NifA 5'-untranslated region was that the cleavage made ribosome-binding sites accessible for translation. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | Q-BETA RNA ; ESCHERICHIA-COLI ; MESSENGER-RNA ; NITROGEN-FIXATION ; HOST FACTOR ; RHODOBACTER-CAPSULATUS ; KLEBSIELLA-PNEUMONIAE ; GLUCOSE-TRANSPORTER ; E-CLEAVAGE ; IN-VITRO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000269959500001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1232]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, YH,Hong, GF. Post-transcriptional regulation of NifA expression by Hfq and RNase E complex in Rhizobium leguminosarum bv. viciae[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(9):719-730. |
| APA | Zhang, YH,&Hong, GF.(2009).Post-transcriptional regulation of NifA expression by Hfq and RNase E complex in Rhizobium leguminosarum bv. viciae.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(9),719-730. |
| MLA | Zhang, YH,et al."Post-transcriptional regulation of NifA expression by Hfq and RNase E complex in Rhizobium leguminosarum bv. viciae".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.9(2009):719-730. |
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