Macropinocytosis contributes to the macrophage foam cell formation in RAW264.7 cells
文献类型：期刊论文
| 作者 | Yao, WQ; Li, K; Liao, K |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2009 |
| 卷号 | 41期号:9页码:773-780 |
| 关键词 | macrophage macropinocytosis foam cell PI3-kinase atherosclerosis |
| 通讯作者 | Liao, K (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn |
| 英文摘要 | The key event in the atherosclerosis development is the lipids uptake by macrophage and the formation of foam cell in subendothelial arterial space. Besides the uptake of modified low-density lipoprotein (LDL) by scavenger receptor-mediated endocytosis, macrophages possess constitutive macropinocytosis, which is capable of taking up a large quantity of solute. Macrophage foam cell formation could be induced in RAW264.7 cells by increasing the serum concentration in the culture medium. Foam cell formation induced by serum could be blocked by phosphoinositide 3-kinase inhibitor, LY294002 or wortmannin, which inhibited macropinocytosis but not receptor-mediated endocytosis. Further analysis indicated that macropinocytosis took place at the gangliosides-enriched membrane area. Cholesterol depletion by beta-methylcyclodextrin-blocked macropinocytosis without affecting scavenger receptor-mediated endocytosis of modified LDLs. These results suggested that macropinocytosis might be one of the important mechanisms for lipid uptake in macrophage. And it made significant contribution to the lipid accumulation and foam cell formation. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | LOW-DENSITY-LIPOPROTEIN ; COLONY-STIMULATING FACTOR ; SCAVENGER RECEPTOR-A ; GROWTH-FACTOR ; IN-VITRO ; M-CSF ; ATHEROSCLEROSIS ; MICE ; MEMBRANE ; PATHWAYS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000269959500007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1234]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yao, WQ,Li, K,Liao, K. Macropinocytosis contributes to the macrophage foam cell formation in RAW264.7 cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(9):773-780. |
| APA | Yao, WQ,Li, K,&Liao, K.(2009).Macropinocytosis contributes to the macrophage foam cell formation in RAW264.7 cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(9),773-780. |
| MLA | Yao, WQ,et al."Macropinocytosis contributes to the macrophage foam cell formation in RAW264.7 cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.9(2009):773-780. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。