Nudel Promotes Axonal Lysosome Clearance and Endo-lysosome Formation via Dynein-Mediated Transport
文献类型：期刊论文
| 作者 | Zhang, QG; Wang, FB; Cao, JL; Shen, YD; Huang, QP; Bao, L; Zhu, X |
| 刊名 | TRAFFIC
 |
| 出版日期 | 2009 |
| 卷号 | 10期号:9页码:1337-1349 |
| 关键词 | axon dynein endosome lysosome mitochondrion neuron Nudel vesicle transport |
| 通讯作者 | Zhu, X (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn |
| 英文摘要 | Axonal transport is critical for neuronal function and survival. Cytoplasmic dynein and its accessory complex dynactin form a microtubule minus end-directed motor in charge of retrograde transport. In this study, we show that Nudel, a dynein regulator, was highly expressed in dorsal root ganglion (DRG) neurons. Microinjection of anti-Nudel antibody into cultured DRG neurons abolished retrograde transport of membranous organelles in the axon and led to dispersions of Golgi cisternae in the soma. As a result, lysosomes, which are normally enriched in the soma, moved persistently into and thus accumulated in axons. Endo-lysosome formation was also markedly delayed. As anterograde motility of mitochondria was not inhibited, the antibody apparently did not abolish retrograde transport by destructing axonal microtubule tracks. Similar results were obtained by microinjecting N-terminal Nudel, anti-dynein antibody or a p150(Glued) mutant capable of abrogating the dynein-dynactin association. These results indicate a critical role of Nudel in dynein-mediated axonal transport. Moreover, the effects of dynein on endolysosome formation and regional sequestration of lysosomes may contribute to defects in the endocytic pathway seen in neurons of patients or animals with malfunction of dynein. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | MOTOR-NEURON DISEASE ; CYTOPLASMIC DYNEIN ; RETROGRADE TRANSPORT ; HIPPOCAMPAL-NEURONS ; DYNACTIN COMPLEX ; MUTANT DYNACTIN ; MIGRATING CELLS ; GROWTH CONE ; PROTEIN ; MICROTUBULES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268794100013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1237]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, QG,Wang, FB,Cao, JL,et al. Nudel Promotes Axonal Lysosome Clearance and Endo-lysosome Formation via Dynein-Mediated Transport[J]. TRAFFIC,2009,10(9):1337-1349. |
| APA | Zhang, QG.,Wang, FB.,Cao, JL.,Shen, YD.,Huang, QP.,...&Zhu, X.(2009).Nudel Promotes Axonal Lysosome Clearance and Endo-lysosome Formation via Dynein-Mediated Transport.TRAFFIC,10(9),1337-1349. |
| MLA | Zhang, QG,et al."Nudel Promotes Axonal Lysosome Clearance and Endo-lysosome Formation via Dynein-Mediated Transport".TRAFFIC 10.9(2009):1337-1349. |
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