A small functional intramolecular region of NodD was identified by mutation
文献类型：期刊论文
| 作者 | Hou, BH; Li, FQ; Yang, XE; Hong, GF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2009 |
| 卷号 | 41期号:10页码:822-830 |
| 关键词 | NodD insertion mutation hinge region |
| 通讯作者 | Hong, GF (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,gfhong@sibs.ac.cn |
| 英文摘要 | In Rhizobium leguminosarum bv. viciae, NodD, as a member of the LysR-type transcriptional regulators (LTTRs), exerts auto-regulation and activates transcription of other nod genes in the presence of naringenin. LTTRs were typically composed of N-terminal DNA-binding domain and C-terminal regulatory domain. In this study, by systematic insertion mutation, a region of 12 amino acids in length of NodD was identified as functional domain. Insertion mutants in this region appeared to acquire the ability of constitutively activating nodA gene and retained their auto-regulation properties. This identified region was shown to be a hinge of NodD as revealed through the model built using Swiss-PDB Viewer software. It is the first time to report that as a member of LysR family, NodD has been shown to contain a short intramolecular domain that influences its performance. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | LEGUMINOSARUM BIOVAR VICIAE ; POLYMERASE ALPHA-SUBUNIT ; DNA-BINDING SITE ; BROAD HOST RANGE ; RHIZOBIUM-LEGUMINOSARUM ; TRANSCRIPTIONAL REGULATOR ; RNA-POLYMERASE ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; CONTACT SITE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000270217400003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1241]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hou, BH,Li, FQ,Yang, XE,et al. A small functional intramolecular region of NodD was identified by mutation[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(10):822-830. |
| APA | Hou, BH,Li, FQ,Yang, XE,&Hong, GF.(2009).A small functional intramolecular region of NodD was identified by mutation.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(10),822-830. |
| MLA | Hou, BH,et al."A small functional intramolecular region of NodD was identified by mutation".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.10(2009):822-830. |
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