Cortactin is involved in transforming growth factor-beta 1-induced epithelial-mesenchymal transition in AML-12 cells
文献类型：期刊论文
| 作者 | Zhang, KH; Wang, DM; Song, JG |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2009 |
| 卷号 | 41期号:10页码:839-845 |
| 关键词 | epithelial-mesenchymal transition cortactin transforming growth factor-beta migration |
| 通讯作者 | Song, JG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,jgsong@sibs.ac.cn |
| 英文摘要 | Cortactin is an F-actin binding protein, regulating cell movement and adhesive junction assembly. However, the function of cortactin in epithelial-mesenchymal transition (EMT) remains elusive. Here we found that during transforming growth factor-beta 1 (TGF-beta 1)-induced EMT in AML-12 murine hepatocytes, cortactin underwent tyrosine dephosphorylation. Inhibition of the dephosphorylation of cortactin by sodium vanadate blocked TGF-beta 1-induced EMT. Knockdown of cortactin by RNAi led to decrease of intercellular junction proteins E-cadherin and Zonula occludens-1 and induced expression of mesenchymal protein fibronectin. Additionally, knockdown of cortactin further promoted TGF-beta 1-induced EMT in AML-12 cells, as determined by EMT markers and cell morphological changes. Moreover, migration assay showed that cortactin knockdown promoted the migration of AML-12 cells, and also enhanced TGF-beta 1-induced migration. Our study showed the involvement of cortactin in the TGF-beta 1-induced EMT. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | TYROSINE PHOSPHORYLATION ; TGF-BETA ; ACTIN POLYMERIZATION ; 11Q13 AMPLIFICATION ; CANCER-CELLS ; N-WASP ; MOTILITY ; INVASION ; OVEREXPRESSION ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000270217400005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1242]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, KH,Wang, DM,Song, JG. Cortactin is involved in transforming growth factor-beta 1-induced epithelial-mesenchymal transition in AML-12 cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(10):839-845. |
| APA | Zhang, KH,Wang, DM,&Song, JG.(2009).Cortactin is involved in transforming growth factor-beta 1-induced epithelial-mesenchymal transition in AML-12 cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(10),839-845. |
| MLA | Zhang, KH,et al."Cortactin is involved in transforming growth factor-beta 1-induced epithelial-mesenchymal transition in AML-12 cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.10(2009):839-845. |
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