Comparative proteomic analysis of cell cycle-dependent apoptosis induced by transforming growth factor-beta
文献类型：期刊论文
| 作者 | Tian, HY; Zhang, KH; Gao, X; Lei, WW; Zhang, L; Yu, ML; Song, JG; Zhao, FK |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
 |
| 出版日期 | 2009 |
| 卷号 | 1794期号:10页码:1387-1397 |
| 关键词 | Proteomics Apoptosis EMT Glutamine synthetase |
| 通讯作者 | Song, JG (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,jgsong@sibs.ac.cn ; fkzhao@sibs.ac.cn |
| 英文摘要 | Transforming growth factor-beta (TGF-beta) can induce G2/M phase-dependent apoptosis and G1/S phase-dependent epithelial-mesenchymal transition (EMT) in hepatocytes, but the underlying mechanism remains poorly understood. In this study, we investigated alterations in the global proteome using two dimensional gel electrophoresis of AML-12 murine hepatocyte cells after treatment with TGF-beta at several time points after synchronization in the G2/M or G1/S phase. Upon TGF-beta treatment, the expression levels of 44 proteins were found to be significantly changed in cells synchronized in the G2/M phase. These proteins were identified by MALDI-TOF/TOF and classified into seven categories according to function. In addition, TGF-beta induced downregulation of glutamine synthetase in cells in G2/M but not G1/S phase, and this was further confirmed by immunoblotting. Moreover, exogenous glutamine completely blocked TGF-beta-incluced apoptosis in G2/M and non-synchronized cells, whereas it had no effect on EMT, suggesting that the downregulation of glutamine synthetase is involved in G2/M phase-dependent apoptosis. These results provide new insight into the mechanism of the multifunctional effects of TGF-beta and how apoptosis and EMT are regulated in the same type of cells. (C) 2009 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | EPITHELIAL-MESENCHYMAL TRANSITION ; NECROSIS-FACTOR-ALPHA ; TGF-BETA ; GLUTAMINE-SYNTHETASE ; CONFERS RESISTANCE ; PROTEIN-KINASE ; EXPRESSION ; HEPATOCYTES ; CANCER ; DEATH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000270259000002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1244]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Tian, HY,Zhang, KH,Gao, X,et al. Comparative proteomic analysis of cell cycle-dependent apoptosis induced by transforming growth factor-beta[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2009,1794(10):1387-1397. |
| APA | Tian, HY.,Zhang, KH.,Gao, X.,Lei, WW.,Zhang, L.,...&Zhao, FK.(2009).Comparative proteomic analysis of cell cycle-dependent apoptosis induced by transforming growth factor-beta.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1794(10),1387-1397. |
| MLA | Tian, HY,et al."Comparative proteomic analysis of cell cycle-dependent apoptosis induced by transforming growth factor-beta".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1794.10(2009):1387-1397. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。