Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication
文献类型：期刊论文
| 作者 | Meng, FL; Hu, Y; Shen, N; Tong, XJ; Wang, JY; Ding, JP; Zhou, JQ |
| 刊名 | EMBO JOURNAL
 |
| 出版日期 | 2009 |
| 卷号 | 28期号:10页码:1466-1478 |
| 关键词 | DNA binding OB-fold Sua5 telomerase telomere |
| 通讯作者 | Zhou, JQ (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci,Grad Sch, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,jqzhou@sibs.ac.cn |
| 英文摘要 | In budding yeast Saccharomyces cerevisiae, telomere length maintenance involves a complicated network as more than 280 telomere maintenance genes have been identified in the nonessential gene deletion mutant set. As a supplement, we identified additional 29 telomere maintenance genes, which were previously taken as essential genes. In this study, we report a novel function of Sua5p in telomere replication. Epistasis analysis and telomere sequencing show that sua5D cells display progressively shortened telomeres at early passages, and Sua5 functions downstream telomerase recruitment. Further, biochemical, structural and genetic studies show that Sua5p specifically binds single-stranded telomeric (ssTG) DNA in vitro through a distinct DNA-binding region on its surface, and the DNA-binding ability is essential for its telomere function. Thus, Sua5p represents a novel ssTG DNA-binding protein and positively regulates the telomere length in vivo. The EMBO Journal (2009) 28, 1466-1478. doi:10.1038/emboj.2009.92; Published online 16 April 2009 |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | SACCHAROMYCES-CEREVISIAE TELOMERASE ; START SITE SELECTION ; GENOME-WIDE SCREEN ; YEAST TELOMERASE ; CATALYTIC SUBUNIT ; CRYSTAL-STRUCTURE ; CHROMOSOME ENDS ; MESSENGER-RNAS ; PIF1P HELICASE ; YRDC PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000266246800010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1251]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Meng, FL,Hu, Y,Shen, N,et al. Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication[J]. EMBO JOURNAL,2009,28(10):1466-1478. |
| APA | Meng, FL.,Hu, Y.,Shen, N.,Tong, XJ.,Wang, JY.,...&Zhou, JQ.(2009).Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication.EMBO JOURNAL,28(10),1466-1478. |
| MLA | Meng, FL,et al."Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication".EMBO JOURNAL 28.10(2009):1466-1478. |
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