Effect of Peptide-to-TiO2 Beads Ratio on Phosphopeptide Enrichment Selectivity
文献类型：期刊论文
| 作者 | Li, QR; Ning, ZB; Tang, JS; Nie, S; Zeng, R |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 8期号:11页码:5375-5381 |
| 关键词 | peptide-to-TiO2 beads ratio phosphorylation enrichment selectivity |
| 通讯作者 | Zeng, R (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Syst Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | Titanium dioxide (TiO2) has been proven to be a highly efficient strategy and widely used for phosphopeptide enrichment. Many advances have been made recently, including online/offline mode and optimization of sample loading/elution buffer; however, beads usage has rarely been explored. In the current study, we found that the peptide-to-TiO2 beads ratio was a significant factor for phosphopeptide enrichment, and insufficient or excessive beads could decrease the selectivity. Specifically, for HeLa total cell lysate, the optimum peptide-to-beads ratio is about 1.2-1.8 (mass/mass) to obtain the highest enrichment selectivity and the maximum phosphopeptides identification with single incubation. Preexperiments are recommended to decide an optimum peptide-to-TiO2 beads ratio when it comes to different samples Interestingly, deficient beads can help identify much more multiphosphorylated peptides than the optimum peptide-to-beads ratio by consecutive incubations. Therefore, if multiphosphorylated peptides identification is desired, deficient beads amount is preferred In addition, consecutive incubation using deficient beads could be used as a fractionation of phosphopeptides besides as an enrichment method. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods |
| 关键词[WOS] | MASS-SPECTROMETRY ; PHOSPHORYLATED PEPTIDES ; TITANIUM-DIOXIDE ; PHOSPHOPROTEOME ANALYSIS ; CHROMATOGRAPHY ; IDENTIFICATION ; AFFINITY ; SEPARATION ; STRATEGIES ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276949600046 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1257]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, QR,Ning, ZB,Tang, JS,et al. Effect of Peptide-to-TiO2 Beads Ratio on Phosphopeptide Enrichment Selectivity[J]. JOURNAL OF PROTEOME RESEARCH,2009,8(11):5375-5381. |
| APA | Li, QR,Ning, ZB,Tang, JS,Nie, S,&Zeng, R.(2009).Effect of Peptide-to-TiO2 Beads Ratio on Phosphopeptide Enrichment Selectivity.JOURNAL OF PROTEOME RESEARCH,8(11),5375-5381. |
| MLA | Li, QR,et al."Effect of Peptide-to-TiO2 Beads Ratio on Phosphopeptide Enrichment Selectivity".JOURNAL OF PROTEOME RESEARCH 8.11(2009):5375-5381. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。