Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation
文献类型：期刊论文
| 作者 | Zhou, J; Zhou, BO; Lenzmeier, BA; Zhou, JQ |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 37期号:11页码:3699-3713 |
| 通讯作者 | Zhou, J (reprint author), Chinese Acad Sci, Chinese Acad Sci, Inst Biochem & Cell Biol, Inst Biol Sci, 320 Yue Yang Rd, Shanghai 2, Peoples R China.,jqzhou@sibs.ac.cn |
| 英文摘要 | In the eukaryotic genome, transcriptionally silent chromatin tends to propagate along a chromosome and encroach upon adjacent active chromatin. The silencing machinery can be stopped by chromatin boundary elements. We performed a screen in Saccharomyces cerevisiae for proteins that may contribute to the establishment of a chromatin boundary. We found that disruption of histone deacetylase Rpd3p results in defective boundary activity, leading to a Sir-dependent local propagation of transcriptional repression. In rpd3 cells, the amount of Sir2p that was normally found in the nucleolus decreased and the amount of Sir2p found at telomeres and at HM and its adjacent loci increased, leading to an extension of silent chromatin in those areas. In addition, Rpd3p interacted directly with chromatin at boundary regions to deacetylate histone H4 at lysine 5 and at lysine 12. Either the mutation of histone H4 at lysine 5 or a decrease in the histone acetyltransferase (HAT) activity of Esa1p abrogated the silencing phenotype associated with rpd3 mutation, suggesting a novel role for the H4 amino terminus in Rpd3p-mediated heterochromatin boundary regulation. Together, these data provide insight into the molecular mechanisms for the anti-silencing functions of Rpd3p during the formation of heterochromatin boundaries. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | SACCHAROMYCES-CEREVISIAE ; GENOME-WIDE ; HETEROCHROMATIN BOUNDARIES ; REPRESSED CHROMATIN ; GENE DELETION ; VARIANT H2A.Z ; YEAST RDNA ; IN-VIVO ; COMPLEX ; ACETYLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267441800027 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1258]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, J,Zhou, BO,Lenzmeier, BA,et al. Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation[J]. NUCLEIC ACIDS RESEARCH,2009,37(11):3699-3713. |
| APA | Zhou, J,Zhou, BO,Lenzmeier, BA,&Zhou, JQ.(2009).Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation.NUCLEIC ACIDS RESEARCH,37(11),3699-3713. |
| MLA | Zhou, J,et al."Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation".NUCLEIC ACIDS RESEARCH 37.11(2009):3699-3713. |
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