Genome-wide identification of target genes repressed by the zinc finger transcription factor REST/NRSF in the HEK 293 cell line
文献类型：期刊论文
| 作者 | Liu, ZH; Liu, M; Niu, G; Cheng, Y; Fei, J |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2009 |
| 卷号 | 41期号:12页码:1008-1017 |
| 关键词 | REST NRSF RE1 NRSE RNAi microarray transcription factor |
| 通讯作者 | Fei, J (reprint author), Shanghai Res Ctr Model Organisms, Shanghai 201210, Peoples R China.,jfei@tongji.edu.cn |
| 英文摘要 | Transcriptional repression is as important as transcriptional activation in establishing cell-type specific patterns of gene expression. RE1-silencing transcription factor (REST), also known as neuronal restrictive silencing factor (NRSF), is a transcriptional regulator that represses a battery of neuronal differentiation genes in non-neuronal cells or in neural progenitor cells by binding to a specific DNA sequence (repressor element-1/neuron-restrictive silencer element, RE1/NRSE). REST/NRSF functions in the neuronal development are widely studied, however, little is known about target genes in various non-neuronal lineages that may result in cell differentiation. Here, we use RNA interference (RNAi) technology combined with the microarray strategy to identify potential REST/NRSF targets and RE1/NRSEs in human non-neuronal cell line HEK 293. Expression of 54 genes was up-regulated by inhibition of REST/NRSF in the HEK 293 cells according to the microarray experiment and 13 of those were further confirmed by quantitative RT-PCR. Our results confirmed the good confidence and reliability of current research data based on in silico, chromatin immunoprecipitation in combination with microarrays (ChIP-chip), and high-throughput sequencing (ChIP-seq). However, in view of the fact that thousands of genes have been testified or predicted to be recognized by REST/NRSF, our data show that only a few genes among those are directly up-regulated by the interaction of REST/NRSF with RE1/NRSEs sites in gene sequences. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | CHIP-SEQ DATA ; NEURON-RESTRICTIVE SILENCER ; IN-VIVO ; BINDING-SITES ; REST ; PROTEIN ; EXPRESSION ; DOMAINS ; ELEMENT ; CANCER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000272463800005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1262]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, ZH,Liu, M,Niu, G,et al. Genome-wide identification of target genes repressed by the zinc finger transcription factor REST/NRSF in the HEK 293 cell line[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(12):1008-1017. |
| APA | Liu, ZH,Liu, M,Niu, G,Cheng, Y,&Fei, J.(2009).Genome-wide identification of target genes repressed by the zinc finger transcription factor REST/NRSF in the HEK 293 cell line.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(12),1008-1017. |
| MLA | Liu, ZH,et al."Genome-wide identification of target genes repressed by the zinc finger transcription factor REST/NRSF in the HEK 293 cell line".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.12(2009):1008-1017. |
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