Different Transcription Factors Regulate nestin Gene Expression during P19 Cell Neural Differentiation and Central Nervous System Development
文献类型：期刊论文
| 作者 | Jin, ZG; Liu, L; Bian, W; Chen, YF; Xu, GL; Cheng, LP; Jing, NH |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2009 |
| 卷号 | 284期号:12页码:8160-8173 |
| 通讯作者 | Cheng, LP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,lpcheng@sibs.ac.cn ; njing@sibs.ac.cn |
| 英文摘要 | Nestin is a molecular marker for neural progenitor cells. Rat and human nestin genes possess a central nervous system-specific enhancer within their second introns. However, the transcription factors that bind to the nestin enhancer have not been fully elucidated. Here, we show that the second intron of the mouse nestin gene is sufficient to drive reporter gene expression in the developing nervous system. The core sequence of this central nervous system-specific enhancer localizes to the 3' 320-bp region. The cis-elements for Sox and POU family transcription factors and the hormone-responsive element are essential for nestin expression during embryonic carcinoma P19 cell neural differentiation and in the developing chick neural tube. Interestingly, different transcription factors bind to the nestin enhancer at different stages of P19 cell neural differentiation and central nervous system development. Sox2 and SF1 may mediate basal nestin expression in undifferentiated P19EC cells, whereas Sox2, Brn1, and Brn2 bind to the enhancer in P19 neural progenitor cells. Similarly, in vivo, Oct1 binds to the nestin enhancer in embryonic day 8.5 (E8.5) mouse embryos, and Oct1, Brn1, and Brn2 bind to this enhancer in E10.5 and E12.5 mouse embryos. Our studies therefore suggest a temporal coordination of transcription factors in determining nestin gene expression. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | INTERMEDIATE-FILAMENT PROTEIN ; EMBRYONIC CARCINOMA-CELLS ; GFP TRANSGENIC MICE ; CNS STEM-CELLS ; PROGENITOR CELLS ; NEURONAL DIFFERENTIATION ; PRECURSOR CELLS ; 2ND INTRON ; IN-VITRO ; PROMOTER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264195600078 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1270]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jin, ZG,Liu, L,Bian, W,et al. Different Transcription Factors Regulate nestin Gene Expression during P19 Cell Neural Differentiation and Central Nervous System Development[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2009,284(12):8160-8173. |
| APA | Jin, ZG.,Liu, L.,Bian, W.,Chen, YF.,Xu, GL.,...&Jing, NH.(2009).Different Transcription Factors Regulate nestin Gene Expression during P19 Cell Neural Differentiation and Central Nervous System Development.JOURNAL OF BIOLOGICAL CHEMISTRY,284(12),8160-8173. |
| MLA | Jin, ZG,et al."Different Transcription Factors Regulate nestin Gene Expression during P19 Cell Neural Differentiation and Central Nervous System Development".JOURNAL OF BIOLOGICAL CHEMISTRY 284.12(2009):8160-8173. |
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