Calcium signaling-induced Smad3 nuclear accumulation induces acetylcholinesterase transcription in apoptotic HeLa cells
文献类型：期刊论文
| 作者 | Gao, W; Zhu, H; Zhang, JY; Zhang, XJ |
| 刊名 | CELLULAR AND MOLECULAR LIFE SCIENCES
 |
| 出版日期 | 2009 |
| 卷号 | 66期号:13页码:2181-2193 |
| 关键词 | Smad3 Acetylcholinesterase Cellular calcium perturbation Apoptosis Nuclear accumulation Transcriptional activation |
| 通讯作者 | Zhang, XJ (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 YueYang Rd, Shanghai 200031, Peoples R China.,xjzhang@sibs.ac.cn |
| 英文摘要 | Recently, acetylcholinesterase (AChE) has been studied as an important apoptosis regulator. We previously showed that cellular calcium mobilization upregulated AChE expression by modulating promoter activity and mRNA stability. In this study, we have identified a potential Smad3/4 binding element, TGCCAGACA, located within the -601 to -571 bp fragment of the AChE promoter, as an important calcium response motif. Smad2/3 and Smad4 were shown to bind this element. Overexpression of human Smad3 increased AChE transcription activity in a dose-dependent manner in HeLa cells, whereas dominant-negative Smad3 blocked this activation. Upon A23187 and thapsigargin treatment, nuclear Smad3 accumulation was observed, an effect that was blocked by the intracellular Ca(2+) chelator BAPTA-AM. Calcium-induced AChE transcriptional activation was significantly blocked when the nuclear localization signal of Smad3 was destroyed. Taken together, our data suggest Smad3 can regulate AChE transcriptional activation following calcium-induced nuclear accumulation. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | MESSENGER-RNA STABILITY ; PRECURSOR PROTEIN GENE ; ALZHEIMERS-DISEASE ; INTRACELLULAR CALCIUM ; EXPRESSION ; THAPSIGARGIN ; BINDING ; INDUCTION ; ACHE ; CALRETICULIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267680500014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1274]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Gao, W,Zhu, H,Zhang, JY,et al. Calcium signaling-induced Smad3 nuclear accumulation induces acetylcholinesterase transcription in apoptotic HeLa cells[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2009,66(13):2181-2193. |
| APA | Gao, W,Zhu, H,Zhang, JY,&Zhang, XJ.(2009).Calcium signaling-induced Smad3 nuclear accumulation induces acetylcholinesterase transcription in apoptotic HeLa cells.CELLULAR AND MOLECULAR LIFE SCIENCES,66(13),2181-2193. |
| MLA | Gao, W,et al."Calcium signaling-induced Smad3 nuclear accumulation induces acetylcholinesterase transcription in apoptotic HeLa cells".CELLULAR AND MOLECULAR LIFE SCIENCES 66.13(2009):2181-2193. |
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