Growth arrest induces primary-cilium formation and sensitizes IGF-1-receptor signaling during differentiation induction of 3T3-L1 preadipocytes
文献类型：期刊论文
| 作者 | Zhu, D; Shi, S; Wang, HZ; Liao, K |
| 刊名 | JOURNAL OF CELL SCIENCE
 |
| 出版日期 | 2009 |
| 卷号 | 122期号:15页码:2760-2768 |
| 关键词 | Primary cilium IGF-1 receptor alpha-tubulin acetylation Growth arrest Adipocyte differentiation 3T3-L1 cell |
| 通讯作者 | Liao, K (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn |
| 英文摘要 | The first stage of 3T3-L1 adipocyte differentiation is growth arrest, which is achieved by contact inhibition at confluence. In growth-arrested confluent 3T3-L1 preadipocytes, alpha-tubulin acetylation and primary-cilium formation were induced. The blockade of primary-cilium formation by suppressing IFT88 or Kif3a inhibited 3T3-L1 adipocyte differentiation. IGF-1 (IGF-I)-receptor signaling, which is essential for differentiation induction, was sensitized by the formation of a primary cilium in confluent 3T3-L1 preadipocytes. The receptor located in primary cilium was more sensitive to insulin stimulation than that not located in cilia. During cilium formation, insulin receptor substrate 1 (IRS-1), one of the important downstream signaling molecules of the IGF-1 receptor, was recruited to the basal body at which it was phosphorylated on tyrosine by the receptor kinase in cilia. Akt-1, an important signal molecule of the IGF-1 receptor in adipocyte differentiation, was also activated at the basal body. These IGF-1-receptor signaling processes were all inhibited in IFT88- or Kif3a-knockdown cells. Thus, the primary cilium and its basal body formed an organized signaling pathway for the IGF-1 receptor to induce adipocyte differentiation in confluent 3T3-L1 preadipocytes. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | ADIPOCYTE DIFFERENTIATION ; FACTOR-I ; LIPID RAFTS ; MOLECULAR REGULATION ; INSULIN ; KINASE ; ACTIVATION ; CILIARY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268179400020 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1275]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, D,Shi, S,Wang, HZ,et al. Growth arrest induces primary-cilium formation and sensitizes IGF-1-receptor signaling during differentiation induction of 3T3-L1 preadipocytes[J]. JOURNAL OF CELL SCIENCE,2009,122(15):2760-2768. |
| APA | Zhu, D,Shi, S,Wang, HZ,&Liao, K.(2009).Growth arrest induces primary-cilium formation and sensitizes IGF-1-receptor signaling during differentiation induction of 3T3-L1 preadipocytes.JOURNAL OF CELL SCIENCE,122(15),2760-2768. |
| MLA | Zhu, D,et al."Growth arrest induces primary-cilium formation and sensitizes IGF-1-receptor signaling during differentiation induction of 3T3-L1 preadipocytes".JOURNAL OF CELL SCIENCE 122.15(2009):2760-2768. |
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