Identification of a novel mouse P4-ATPase family member highly expressed during spermatogenesis
文献类型：期刊论文
| 作者 | Xu, P; Okkeri, J; Hanisch, S; Hu, RY; Xu, Q; Pomorski, TG; Ding, XY |
| 刊名 | JOURNAL OF CELL SCIENCE
 |
| 出版日期 | 2009 |
| 卷号 | 122期号:16页码:2866-2876 |
| 关键词 | P-type ATPase Aminophospholipid translocase Acrosome biogenesis Phospholipid asymmetry Spermatogenesis |
| 通讯作者 | Ding, XY (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Key Lab Stem Cell Biol,Grad Sch, Shanghai Inst Biol Sci,Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,tgp@life.ku.dk ; xyding@sunm.shcnc.ac.cn |
| 英文摘要 | P4-ATPases are transmembrane proteins unique to eukaryotes that play a fundamental role in vesicular transport. They have been proposed to act as phospholipid flippases thereby regulating lipid topology in cellular membranes. We cloned and characterized a novel murine P4-ATPase that is specifically expressed in testis, and named it FetA (flippase expressed in testis splicing form A). When expressed in Saccharomyces cerevisiae, FetA localizes partially to the plasma membrane resulting in increased internalization of NBD-labeled phosphatidylethanolamine and phosphatidylcholine, supporting a role for FetA in the inward lipid translocation across cellular membranes. In mouse testis, FetA protein is detected in gamete cells, from pachytene spermatocytes to mature sperms, and its intracellular localization is tightly related with acrosome formation, a process that involves intensive intracellular vesicle formation and fusion. Furthermore, loss-of-function of FetA by RNA interference in mastocytoma P815 cells profoundly perturbs the structural organization of the Golgi complex and causes loss of constitutive secretion at lower temperature. Our findings point to an essential role of FetA in Golgi morphology and secretory function, suggesting a crucial role for this novel murine P4-ATPase in spermatogenesis. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | P-TYPE ATPASES ; YEAST PLASMA-MEMBRANE ; PHOSPHOLIPID TRANSLOCATION ; AMINOPHOSPHOLIPID TRANSLOCASES ; GOLGI-COMPLEX ; IN-VIVO ; ASYMMETRY ; SUBFAMILY ; TRANSPORT ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268727400010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1278]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, P,Okkeri, J,Hanisch, S,et al. Identification of a novel mouse P4-ATPase family member highly expressed during spermatogenesis[J]. JOURNAL OF CELL SCIENCE,2009,122(16):2866-2876. |
| APA | Xu, P.,Okkeri, J.,Hanisch, S.,Hu, RY.,Xu, Q.,...&Ding, XY.(2009).Identification of a novel mouse P4-ATPase family member highly expressed during spermatogenesis.JOURNAL OF CELL SCIENCE,122(16),2866-2876. |
| MLA | Xu, P,et al."Identification of a novel mouse P4-ATPase family member highly expressed during spermatogenesis".JOURNAL OF CELL SCIENCE 122.16(2009):2866-2876. |
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