Activation of Phosphatidylinositol-Linked D-1-Like Receptor Modulates FGF-2 Expression in Astrocytes via IP3-Dependent Ca2+ Signaling
文献类型：期刊论文
| 作者 | Zhang, XH; Zhou, Z; Wang, DK; Li, AQ; Yin, YQ; Gu, XS; Ding, F; Zhen, XC; Zhou, JW |
| 刊名 | JOURNAL OF NEUROSCIENCE
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| 出版日期 | 2009 |
| 卷号 | 29期号:24页码:7766-7775 |
| 通讯作者 | Zhou, JW (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, State Key Lab Neurosci, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,xczhen@mail.shcnc.ac.cn ; jwzhou@ion.ac.cn |
| 英文摘要 | Fibroblast growth factor-2 (FGF-2) is predominantly synthesized and secreted by astrocytes in adult brain. Our previous study showed that activation of classical dopamine receptor D-1 or D-2 elicits FGF-2 biosynthesis and secretion in astrocytes. Here, we report that astrocytic FGF-2 expression is also regulated by phosphatidylinositol (PI)-linked D-1-like receptor. SKF83959, a selective PI-linked D-1-like receptor agonist, upregulates the levels of FGF-2 protein in striatal astrocyte cultures in classical dopamine D-1 and D-2 receptor-independent manner. The conditional medium derived from SKF83959-activated astrocytes promoted the number of TH+ neurons in vitro. Treatment of astrocytes with SKF83959 increased intracellular calcium in two phases. Inhibition of intracellular calcium oscillation by inositol 1,4,5-triphosphate (IP3) inhibitors blocked the SKF83959-induced increase in FGF-2 expression. Moreover, intraperitoneal administration of SKF83959 reversed l-methyl-4-phenyl-l,2,3,6-tetrahydropypridine (MPTP)-induced reduction in FGF-2 expression in both the striatum and ventral midbrain and resulted in marked protection of dopaminergic neurons from MPTP-induced neurotoxicity. These results indicate that IP3/Ca2+/calmodulin-dependent protein kinase is an uncharted intracellular signaling pathway that is crucial for the regulation of FGF-2 synthesis in astrocytes. PI-linked D-1-like receptor plays an important role in the regulation of astrocytic FGF-2 expression and neuroprotection which may provide a potential target for the drug discovery in Parkinson's disease. |
| 学科主题 | Neurosciences & Neurology |
| 类目[WOS] | Neurosciences |
| 关键词[WOS] | D-1 DOPAMINE AGONISTS ; FIBROBLAST-GROWTH-FACTOR ; PARKINSONS-DISEASE ; VARYING EFFICACIES ; RAT-BRAIN ; SUBSTANTIA-NIGRA ; SKF 83959 ; NEURONS ; SKF83959 ; CALCIUM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267131000016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1286]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, XH,Zhou, Z,Wang, DK,et al. Activation of Phosphatidylinositol-Linked D-1-Like Receptor Modulates FGF-2 Expression in Astrocytes via IP3-Dependent Ca2+ Signaling[J]. JOURNAL OF NEUROSCIENCE,2009,29(24):7766-7775. |
| APA | Zhang, XH.,Zhou, Z.,Wang, DK.,Li, AQ.,Yin, YQ.,...&Zhou, JW.(2009).Activation of Phosphatidylinositol-Linked D-1-Like Receptor Modulates FGF-2 Expression in Astrocytes via IP3-Dependent Ca2+ Signaling.JOURNAL OF NEUROSCIENCE,29(24),7766-7775. |
| MLA | Zhang, XH,et al."Activation of Phosphatidylinositol-Linked D-1-Like Receptor Modulates FGF-2 Expression in Astrocytes via IP3-Dependent Ca2+ Signaling".JOURNAL OF NEUROSCIENCE 29.24(2009):7766-7775. |
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