Structural Basis for Ubiquitin Recognition by a Novel Domain from Human Phospholipase A(2)-activating Protein
文献类型：期刊论文
| 作者 | Fu, QS; Zhou, CJ; Gao, HC; Jiang, YJ; Zhou, ZR; Hong, J; Yao, WM; Song, AX; Lin, DH; Hu, HY |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2009 |
| 卷号 | 284期号:28页码:19043-19052 |
| 通讯作者 | Lin, DH (reprint author), Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China.,dhlin@mail.shcnc.ac.cn |
| 英文摘要 | Ubiquitin (Ub) is an essential modifier conserved in all eukaryotes from yeast to human. Phospholipase A(2)-activating protein (PLAA), a mammalian homolog of yeast DOA1/UFD3, has been proposed to be able to bind with Ub, which plays important roles in endoplasmic reticulum-associated degradation, vesicle formation, and DNA damage response. We have identified a core domain from the PLAA family ubiquitin-binding region of human PLAA (residues 386-465, namely PFUC) that can bind Ub and elucidated its solution structure and Ub-binding mode by NMR approaches. The PFUC domain possesses equal population of two conformers in solution by cis/trans-isomerization, whereas the two isomers exhibit almost equivalent Ub binding abilities. This domain structure takes a novel fold consisting of four beta-strands and two alpha-helices, and the Ub-binding site on PFUC locates in the surface of alpha 2-helix, which is to some extent analogous to those of UBA, CUE, and UIM domains. This study provides structural basis and biochemical information for Ub recognition of the novel PFU domain from a PLAA family protein that may connect ubiquitination and degradation in endoplasmic reticulum-associated degradation. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | INTERACTING MOTIF ; CHEMICAL-SHIFT ; SACCHAROMYCES-CEREVISIAE ; CRYSTAL-STRUCTURE ; BINDING DOMAINS ; COMPLEX REVEALS ; PROTEASOME ; ERAD ; NMR ; S5A |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267711500053 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1287]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Fu, QS,Zhou, CJ,Gao, HC,et al. Structural Basis for Ubiquitin Recognition by a Novel Domain from Human Phospholipase A(2)-activating Protein[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2009,284(28):19043-19052. |
| APA | Fu, QS.,Zhou, CJ.,Gao, HC.,Jiang, YJ.,Zhou, ZR.,...&Hu, HY.(2009).Structural Basis for Ubiquitin Recognition by a Novel Domain from Human Phospholipase A(2)-activating Protein.JOURNAL OF BIOLOGICAL CHEMISTRY,284(28),19043-19052. |
| MLA | Fu, QS,et al."Structural Basis for Ubiquitin Recognition by a Novel Domain from Human Phospholipase A(2)-activating Protein".JOURNAL OF BIOLOGICAL CHEMISTRY 284.28(2009):19043-19052. |
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