Regulation of Immature Dendritic Cell Migration by RhoA Guanine Nucleotide Exchange Factor Arhgef5
文献类型：期刊论文
| 作者 | Wang, ZL; Kumamoto, Y; Wang, P; Gan, XQ; Lehmann, D; Smrcka, AV; Cohn, L; Iwasaki, A; Li, L; Wu, DQ |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2009 |
| 卷号 | 284期号:42页码:28599-28606 |
| 通讯作者 | Li, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,lli@sibs.ac.cn ; Dan.Wu@Yale.edu |
| 英文摘要 | There are a large number of Rho guanine nucleotide exchange factors, most of which have no known functions. Here, we carried out a short hairpin RNA-based functional screen of Rho-GEFs for their roles in leukocyte chemotaxis and identified Arhgef5 as an important factor in chemotaxis of a macrophage phage-like RAW264.7 cell line. Arhgef5 can strongly activate RhoA and RhoB and weakly RhoC and RhoG, but not Rac1, RhoQ, RhoD, or RhoV, in transfected human embryonic kidney 293 cells. In addition, G beta gamma interacts with Arhgef5 and can stimulate Arhgef5-mediated activation of RhoA in an in vitro assay. In vivo roles of Arhgef5 were investigated using an Arhgef-5-null mouse line. Arhgef5 deficiency did not affect chemotaxis of mouse macrophages, T and B lymphocytes, and bone marrow-derived mature dendritic cells (DC), but it abrogated MIP1 alpha-induced chemotaxis of immature DCs and impaired migration of DCs from the skin to lymph node. In addition, Arhgef5 deficiency attenuated allergic airway inflammation. Therefore, this study provides new insights into signaling mechanisms for DC migration regulation. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | FAMILY GTPASES ; BONE-MARROW ; ACTIVATION ; PROTEIN ; RAC ; CDC42 ; P-REX1 ; NEUTROPHILS ; GEF ; ORGANIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000270678900014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1298]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, ZL,Kumamoto, Y,Wang, P,et al. Regulation of Immature Dendritic Cell Migration by RhoA Guanine Nucleotide Exchange Factor Arhgef5[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2009,284(42):28599-28606. |
| APA | Wang, ZL.,Kumamoto, Y.,Wang, P.,Gan, XQ.,Lehmann, D.,...&Wu, DQ.(2009).Regulation of Immature Dendritic Cell Migration by RhoA Guanine Nucleotide Exchange Factor Arhgef5.JOURNAL OF BIOLOGICAL CHEMISTRY,284(42),28599-28606. |
| MLA | Wang, ZL,et al."Regulation of Immature Dendritic Cell Migration by RhoA Guanine Nucleotide Exchange Factor Arhgef5".JOURNAL OF BIOLOGICAL CHEMISTRY 284.42(2009):28599-28606. |
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