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Mechanoenzymatic Cleavage of the Ultralarge Vascular Protein von Willebrand Factor
文献类型：期刊论文
| 作者 | Zhang, XH; Halvorsen, K; Zhang, CZ; Wong, WP; Springer, TA |
| 刊名 | SCIENCE
 |
| 出版日期 | 2009 |
| 卷号 | 324期号:5932页码:1330-1334 |
| 通讯作者 | Springer, TA (reprint author), Harvard Univ, Sch Med, Immune Dis Inst, Boston, MA 02115 USA.,wong@rowland.harvard.edu ; springer@idi.harvard.edu |
| 英文摘要 | Von Willebrand factor (VWF) is secreted as ultralarge multimers that are cleaved in the A2 domain by the metalloprotease ADAMTS13 to give smaller multimers. Cleaved VWF is activated by hydrodynamic forces found in arteriolar bleeding to promote hemostasis, whereas uncleaved VWF is activated at lower, physiologic shear stresses and causes thrombosis. Single-molecule experiments demonstrate that elongational forces in the range experienced by VWF in the vasculature unfold the A2 domain, and only the unfolded A2 domain is cleaved by ADAMTS13. In shear flow, tensile force on a VWF multimer increases with the square of multimer length and is highest at the middle, providing an efficient mechanism for homeostatic regulation of VWF size distribution by force-induced A2 unfolding and cleavage by ADAMTS13, as well as providing a counterbalance for VWF-mediated platelet aggregation. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | FACTOR A2 DOMAIN ; VONWILLEBRAND-FACTOR ; FACTOR MULTIMERS ; SHEAR-STRESS ; PLASMA ; ADAMTS13 ; SUBSTRATE ; ADHESION ; DISEASE ; CONFORMATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000266635100047 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1302]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, XH,Halvorsen, K,Zhang, CZ,et al. Mechanoenzymatic Cleavage of the Ultralarge Vascular Protein von Willebrand Factor[J]. SCIENCE,2009,324(5932):1330-1334. |
| APA | Zhang, XH,Halvorsen, K,Zhang, CZ,Wong, WP,&Springer, TA.(2009).Mechanoenzymatic Cleavage of the Ultralarge Vascular Protein von Willebrand Factor.SCIENCE,324(5932),1330-1334. |
| MLA | Zhang, XH,et al."Mechanoenzymatic Cleavage of the Ultralarge Vascular Protein von Willebrand Factor".SCIENCE 324.5932(2009):1330-1334. |
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