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Mechanoenzymatic Cleavage of the Ultralarge Vascular Protein von Willebrand Factor

文献类型:期刊论文

作者Zhang, XH; Halvorsen, K; Zhang, CZ; Wong, WP; Springer, TA
刊名SCIENCE
出版日期2009
卷号324期号:5932页码:1330-1334
通讯作者Springer, TA (reprint author), Harvard Univ, Sch Med, Immune Dis Inst, Boston, MA 02115 USA.,wong@rowland.harvard.edu ; springer@idi.harvard.edu
英文摘要Von Willebrand factor (VWF) is secreted as ultralarge multimers that are cleaved in the A2 domain by the metalloprotease ADAMTS13 to give smaller multimers. Cleaved VWF is activated by hydrodynamic forces found in arteriolar bleeding to promote hemostasis, whereas uncleaved VWF is activated at lower, physiologic shear stresses and causes thrombosis. Single-molecule experiments demonstrate that elongational forces in the range experienced by VWF in the vasculature unfold the A2 domain, and only the unfolded A2 domain is cleaved by ADAMTS13. In shear flow, tensile force on a VWF multimer increases with the square of multimer length and is highest at the middle, providing an efficient mechanism for homeostatic regulation of VWF size distribution by force-induced A2 unfolding and cleavage by ADAMTS13, as well as providing a counterbalance for VWF-mediated platelet aggregation.
学科主题Science & Technology - Other Topics
类目[WOS]Multidisciplinary Sciences
关键词[WOS]FACTOR A2 DOMAIN ; VONWILLEBRAND-FACTOR ; FACTOR MULTIMERS ; SHEAR-STRESS ; PLASMA ; ADAMTS13 ; SUBSTRATE ; ADHESION ; DISEASE ; CONFORMATION
收录类别SCI
语种英语
WOS记录号WOS:000266635100047
版本出版稿
源URL[http://202.127.25.143/handle/331003/1302]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Zhang, XH,Halvorsen, K,Zhang, CZ,et al. Mechanoenzymatic Cleavage of the Ultralarge Vascular Protein von Willebrand Factor[J]. SCIENCE,2009,324(5932):1330-1334.
APA Zhang, XH,Halvorsen, K,Zhang, CZ,Wong, WP,&Springer, TA.(2009).Mechanoenzymatic Cleavage of the Ultralarge Vascular Protein von Willebrand Factor.SCIENCE,324(5932),1330-1334.
MLA Zhang, XH,et al."Mechanoenzymatic Cleavage of the Ultralarge Vascular Protein von Willebrand Factor".SCIENCE 324.5932(2009):1330-1334.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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