Role of SIP30 in the development and maintenance of peripheral nerve injury-induced neuropathic pain
文献类型：期刊论文
| 作者 | Zhang, YQ; Guo, N; Peng, GD; Han, M; Raincrow, J; Chiu, CH; Coolen, LM; Wenthold, RJ; Zhao, ZQ; Jing, NH |
| 刊名 | PAIN
 |
| 出版日期 | 2009 |
| 卷号 | 146期号:1页码:130-140 |
| 关键词 | SIP30 Neuropathic pain Chronic constriction injury Spinal cord Intrathecal Dorsal horn |
| 通讯作者 | Yu, L (reprint author), Rutgers State Univ, Dept Genet, 607 Allison Rd, Piscataway, NJ 08854 USA.,lei.yu@rutgers.edu |
| 英文摘要 | Using the chronic constriction injury (CCI) model of neuropathic pain, we profiled gene expression in the rat spinal cord, and identified SIP30 as a gene whose expression was elevated after CCI. SIP30 was previously shown to interact with SNAP25, but whose function was otherwise unknown. We now show that in the spinal cord, SIP30 was present in the dorsal horn laminae where the peripheral nociceptive inputs first synapse, co-localizing with nociception-related neuropeptides CGRP and substance P. With the onset of neuropathic pain after CCI surgery, SIP30 mRNA and protein levels increased in the ipsilateral side of the spinal cord, suggesting a potential association between SIP30 and neuropathic pain. When CCI-upregulated SIP30 was inhibited by intrathecal antisense oligonucleotide administration, neuropathic pain was attenuated. This neuropathic pain-reducing effect was observed both during neuropathic pain onset following CCI, and after neuropathic pain was fully established, implicating SIP30 involvement in the development and maintenance phases of neuropathic pain. Using a secretion assay in PC12 cells, anti-SIP30 siRNA decreased the total pool of synaptic vesicles available for exocytosis, pointing to a potential function for SIP30. These results suggest a role of SIP30 in the development and maintenance of peripheral nerve injury-induced neuropathic pain. (C) 2009 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved. |
| 学科主题 | Anesthesiology; Neurosciences & Neurology |
| 类目[WOS] | Anesthesiology ; Clinical Neurology ; Neurosciences |
| 关键词[WOS] | CA2+-DEPENDENT EXOCYTOSIS ; GENE-EXPRESSION ; FUNCTIONAL INVOLVEMENT ; C57BL/6 MICE ; PROTEIN ; SNAP-25 ; SYNTAXIN ; RAT ; SNARE ; IDENTIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000271781600023 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1305]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, YQ,Guo, N,Peng, GD,et al. Role of SIP30 in the development and maintenance of peripheral nerve injury-induced neuropathic pain[J]. PAIN,2009,146(1):130-140. |
| APA | Zhang, YQ.,Guo, N.,Peng, GD.,Han, M.,Raincrow, J.,...&Yu, L.(2009).Role of SIP30 in the development and maintenance of peripheral nerve injury-induced neuropathic pain.PAIN,146(1),130-140. |
| MLA | Zhang, YQ,et al."Role of SIP30 in the development and maintenance of peripheral nerve injury-induced neuropathic pain".PAIN 146.1(2009):130-140. |
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