Prospero-related homeobox protein (Prox1) inhibits hepatitis B virus replication through repressing multiple cis regulatory elements
文献类型：期刊论文
| 作者 | Qin, J; Zhai, JW; Hong, R; Shan, SF; Kong, YY; Wen, YM; Wang, Y; Liu, J; Xie, YH |
| 刊名 | JOURNAL OF GENERAL VIROLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 90期号:1页码:1246-1255 |
| 通讯作者 | Liu, J (reprint author), Fudan Univ, Shanghai Med Coll, Inst Med Microbiol, Key Lab Med Mol Virol, Shanghai 200032, Peoples R China.,liujing@sibs.ac.cn ; yhxie@sibs.ac.cn |
| 英文摘要 | Hepatitis B virus (HBV) gene transcription is controlled by viral promoters and enhancers, the activities of which are regulated by a number of cellular factors as well as virally encoded proteins. Negative regulation of HBV cis-element activities by cellular factors has been reported less widely than their activation. In this study, we report that nuclear factor Pros pero-related homeobox protein (Prox1) represses HBV antigen expression and genome replication in cultured hepatocytes. By using reporter-gene analysis, three of the four HBV promoters, namely the enhancer II/core promoter (ENII/Cp), preS1 promoter (Sp1) and enhancer I/X promoter, were identified as targets for Prox1-mediated repression. Mechanistic analysis then revealed that, for ENII/Cp, Prox1 serves as a corepressor of liver receptor homologue 1 (LRH-1) and downregulates LRH-1-mediated activation of ENII/Cp, whereas for Sp1, Prox1 partially represses Spl activity by interacting directly with hepatocyte nuclear factor 1. Identification of Prox1 as an HBV repressor will help in the understanding of detailed interactions between viral cis elements and host cellular factors and may also form the basis for new anti-HBV intervention therapeutics. |
| 学科主题 | Biotechnology & Applied Microbiology; Virology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Virology |
| 关键词[WOS] | NUCLEAR RECEPTOR SUPERFAMILY ; X PROMOTER ACTIVITY ; TRANSCRIPTION FACTOR ; ENHANCER-II ; EF-C ; BINDING ; LIVER ; GENE ; DOMAIN ; CONSERVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000266019700022 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1313]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Qin, J,Zhai, JW,Hong, R,et al. Prospero-related homeobox protein (Prox1) inhibits hepatitis B virus replication through repressing multiple cis regulatory elements[J]. JOURNAL OF GENERAL VIROLOGY,2009,90(1):1246-1255. |
| APA | Qin, J.,Zhai, JW.,Hong, R.,Shan, SF.,Kong, YY.,...&Xie, YH.(2009).Prospero-related homeobox protein (Prox1) inhibits hepatitis B virus replication through repressing multiple cis regulatory elements.JOURNAL OF GENERAL VIROLOGY,90(1),1246-1255. |
| MLA | Qin, J,et al."Prospero-related homeobox protein (Prox1) inhibits hepatitis B virus replication through repressing multiple cis regulatory elements".JOURNAL OF GENERAL VIROLOGY 90.1(2009):1246-1255. |
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