Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts
文献类型：期刊论文
| 作者 | Feng, SM; Deng, LF; Chen, W; Shao, JZ; Xu, GL; Li, YP |
| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2009 |
| 卷号 | 417期号:1页码:195-203 |
| 关键词 | acidification Atp6v1c1 (C1) bone resorption filamentous actin (F-actin) ring osteoclast vacuolar-type proton-translocating ATPase (V-ATPase) |
| 通讯作者 | Li, YP (reprint author), Zhejiang Univ, Life Sci Coll, 388 Yuhang Rd, Hangzhou 310058, Peoples R China.,ypli@forsyth.org |
| 英文摘要 | Bone resorption relies oil the extracellular acidification function of V-ATPase (vacuolar-type proton-translocating ATPase) proton pump(s) present in the plasma membrane of osteoclasts. The exact configuration of the osteoclast-specific ruffled border V-ATPases remains largely unknown. In the present study, we found that the V-ATPase Subunit Atp6v1c1 (C1) is highly expressed in osteoclasts, whereas Subunits Atp6v1c2a (C2a) and Atp6v1c2b (C2b) are not. The expression level of C I is highly induced by RANKL [receptor activator for NF-kappa B (nuclear factor kappa B) ligand] during osteoclast differentiation; C1 interacts with Atp6v0a3 (a3) and is mainly localized on the ruffled border of activated osteoclasts. The results of the present study show for the first time that C1-silencing by lentivirus-mediated RNA interference severely impaired osteoclast acidification activity and bone resorption, whereas cell differentiation did not appear to be affected, which is similar to a3 silencing. The F-actin (filamentous actin) ring formation was severely defected in C1-depleted osteoclasts but not in a3-depleted and a3(-/-) osteoclasts. C1 co-localized with microtubules in the plasma membrane and its vicinity in mature osteoclasts. In addition, C1 co-localized with F-actin in the cytoplasm; however, the co-localization chiefly shifted to the cell periphery of Mature osteoclasts. The present study demonstrates that Atp6v1c1 is all essential component of the osteoclast proton pump at the osteoclast ruffled border and that it may regulate F-actin ring formation in osteoclast activation. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | VACUOLAR H+-ATPASE ; MEDIATED EXTRACELLULAR ACIDIFICATION ; V-ATPASE ; SUBUNIT ISOFORMS ; BONE-RESORPTION ; C-SUBUNIT ; RESORBING OSTEOCLASTS ; PLASMA-MEMBRANE ; CROSS-LINKING ; A3 ISOFORM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000262230700019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1315]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Feng, SM,Deng, LF,Chen, W,et al. Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts[J]. BIOCHEMICAL JOURNAL,2009,417(1):195-203. |
| APA | Feng, SM,Deng, LF,Chen, W,Shao, JZ,Xu, GL,&Li, YP.(2009).Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts.BIOCHEMICAL JOURNAL,417(1),195-203. |
| MLA | Feng, SM,et al."Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts".BIOCHEMICAL JOURNAL 417.1(2009):195-203. |
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