Spread of recombinant Autographa californica nucleopolyhedrovirus in various tissues of silkworm Bombyx mori determined by real-time PCR
文献类型：期刊论文
| 作者 | Zhang, Y; Tian, B; Xia, H; Guo, T; Wang, J; Wang, S; Wei, Z; Lu, C |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2008 |
| 卷号 | 373期号:1页码:147-153 |
| 关键词 | real-time PCR quantification viral infection silkworm tissues replication of virus rAcNPV |
| 通讯作者 | Lu, C (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,cdlu@sibs.ac.cn |
| 英文摘要 | A cassette harboring luciferase reporter driven by Bombyx mori A3 promoter was transferred to the bacmid Ac Delta EGT to generate the recombinant virus AcNPVA3Luc (where Ac represents Autographa californica, NPV represents nucleopolyhedrovirus, and A3Luc represents the firefly luciferase reporter cassette driven by the A3 promoter). Recombinant baculovirus was injected into the hemocoele of newly ecdysed fifth instar larvae of the silkworm. The infection of virus in various silkworm tissues was determined by real-time PCR. The profile of viral infection showed that the copy number of recombinant AcNPV (rAcNPV) increased the fastest in the hemocyte, followed by the fat body, Malpighian tubule, middle gut, and silk gland. Detecting in nonpermissive strain silkworm showed that there was no significant difference in the entry of rAcNPV into all tested tissues. The difference in viral infection reflected mainly the big difference in replication of rAcNPV in various tissues of silkworm larvae. Real-time quantitative RT-PCR showed that it was due to the different expression of genes involved in viral DNA replication. (c) 2007 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 关键词[WOS] | NUCLEAR POLYHEDROSIS-VIRUS ; POLYMERASE-CHAIN-REACTION ; TITER DETERMINATION ; INFECTION PROCESS ; SIGNAL PEPTIDE ; COPY-NUMBER ; BACULOVIRUS ; GENE ; INSECT ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000252069000018 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1319]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Y,Tian, B,Xia, H,et al. Spread of recombinant Autographa californica nucleopolyhedrovirus in various tissues of silkworm Bombyx mori determined by real-time PCR[J]. ANALYTICAL BIOCHEMISTRY,2008,373(1):147-153. |
| APA | Zhang, Y.,Tian, B.,Xia, H.,Guo, T.,Wang, J.,...&Lu, C.(2008).Spread of recombinant Autographa californica nucleopolyhedrovirus in various tissues of silkworm Bombyx mori determined by real-time PCR.ANALYTICAL BIOCHEMISTRY,373(1),147-153. |
| MLA | Zhang, Y,et al."Spread of recombinant Autographa californica nucleopolyhedrovirus in various tissues of silkworm Bombyx mori determined by real-time PCR".ANALYTICAL BIOCHEMISTRY 373.1(2008):147-153. |
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