The microtubule plus end-binding protein EB1 is involved in Sertoli cell plasticity in testicular seminiferous tubules
文献类型：期刊论文
| 作者 | Wang, FB; Zhang, QG; Cao, JL; Huang, QP; Zhu, XD |
| 刊名 | EXPERIMENTAL CELL RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 314期号:1页码:213-226 |
| 关键词 | sertoli cell EB1 microtubule testis lentivirus |
| 通讯作者 | Zhu, XD (reprint author), Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn |
| 英文摘要 | Sertoli cells of testis belong to a unique type of polarized epithelial cells and are essential for spermatogenesis. They form the blood-testis barrier at the base of seminiferous tubule. Their numerous long, microtubule-rich processes extend inward and associate with developing germ cells to sustain germ cell growth and differentiation. How Sertoli cells develop and maintain their elaborate processes has been an intriguing question. Here we showed that, by microinjecting lentiviral preparations into mouse testes of 29 days postpartum, we were able to specifically label individual Sertoli cells with GFP, thus achieving a clear view of their natural configurations together with associated germ cells in situ. Moreover, compared to other microtubule plus end-tracking proteins such as CLIP-170 and p150(Glued), EB1 was highly expressed in Sertoli cells and located along microtubule bundles in Sertoli cell processes. Stable overexpression of a GFP-tagged dominant-negative EB1 mutant disrupted microtubule organizations in cultured Sertoli cells. Furthermore, its overexpression in testis Sertoli cells altered their shapes. Sertoli cells in situ became rod-like, with decreased basal and lateral cell processes. Seminiferous tubule circularity and germ cell number were also reduced. These data indicate a requirement of proper microtubule arrays for Sertoli cell plasticity and function in testis. (C) 2007 Elsevier Inc. All rights reserved. |
| 学科主题 | Oncology; Cell Biology |
| 类目[WOS] | Oncology ; Cell Biology |
| 关键词[WOS] | ADENOMATOUS POLYPOSIS-COLI ; MOUSE TESTIS ; ECTOPLASMIC SPECIALIZATION ; SPERMATOGENIC CELLS ; EPITHELIAL-CELLS ; RAT TESTIS ; COLCHICINE ; RECONSTRUCTION ; CONFIGURATION ; DISSOCIATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251663000020 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1326]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, FB,Zhang, QG,Cao, JL,et al. The microtubule plus end-binding protein EB1 is involved in Sertoli cell plasticity in testicular seminiferous tubules[J]. EXPERIMENTAL CELL RESEARCH,2008,314(1):213-226. |
| APA | Wang, FB,Zhang, QG,Cao, JL,Huang, QP,&Zhu, XD.(2008).The microtubule plus end-binding protein EB1 is involved in Sertoli cell plasticity in testicular seminiferous tubules.EXPERIMENTAL CELL RESEARCH,314(1),213-226. |
| MLA | Wang, FB,et al."The microtubule plus end-binding protein EB1 is involved in Sertoli cell plasticity in testicular seminiferous tubules".EXPERIMENTAL CELL RESEARCH 314.1(2008):213-226. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。