Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice
文献类型：期刊论文
| 作者 | Ren, JK; Wang, L; Liu, GX; Zhang, W; Sheng, ZJ; Wang, ZG; Fei, J |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:2页码:111-115 |
| 关键词 | transgenic mice polyclonal antibody EGFP |
| 通讯作者 | Fei, J (reprint author), Shanghai Nan Fang Model Organism Res Ctr, Shanghai 201203, Peoples R China.,jfei@mail.tongji.edu.cn |
| 英文摘要 | Recombinant fusion protein is widely used as an antigen to raise antibodies against the epitope of a target protein. However, the concomitant anticarrier antibody in resulting antiserum reduces the production of the desired antibody and brings about unwanted non-specific immune reactions. It is proposed that the carrier protein transgenic animal could be used to solve this problem. To validate this hypothesis, enhanced green fluorescent protein (EGFP) transgenic mice were produced. By immunizing the mice with fusion protein His6HAtag-EGFP, we showed that the antiserum from the transgenic mice had higher titer antibody against His6HA tag and lower titer antibody against EGFP compared with that from wild-type mice. Therefore, this report describes an improved method to raise high titer antipeptide polyclonal antibody using EGFP transgenic mice that could have application potential in antibody preparation. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | RECOMBINANT PROTEIN ; PEPTIDES ; IMMUNIZATION ; VACCINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253501900003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1336]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ren, JK,Wang, L,Liu, GX,et al. Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(2):111-115. |
| APA | Ren, JK.,Wang, L.,Liu, GX.,Zhang, W.,Sheng, ZJ.,...&Fei, J.(2008).Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(2),111-115. |
| MLA | Ren, JK,et al."Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.2(2008):111-115. |
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