Genetically engineered K cells provide sufficient insulin to correct hyperglycemia in a nude murine model
文献类型：期刊论文
| 作者 | Zhang, YQ; Yao, LQ; Shen, KT; Xu, MD; Zhou, PH; Yang, WG; Liu, XY; Qin, XY |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:2页码:149-157 |
| 关键词 | type 1 diabetes mellitus insulin gene therapy glucose-dependent insulinotropic polypeptide STC-1 cell glucose |
| 通讯作者 | Qin, XY (reprint author), Fudan Univ, Zhongshan Hosp, Dept Gen Surg, Shanghai 200032, Peoples R China.,xyqin@zshospital.com |
| 英文摘要 | A gene therapy-based treatment of type 1 diabetes mellitus requires the development of a surrogate beta cell that can synthesize and secrete functionally active insulin in response to physiologically relevant changes in ambient glucose levels. In this study, the murine enteroendocrine cell line STC-1 was genetically modified by stable transfection. Two clone cells were selected (STC-1-2 and STC-1-14) that secreted the highest levels of insulin among the 22 clones expressing insulin from 0 to 157.2 mu IU/ml/10(6) cells/d. After glucose concentration in the culture medium was increased from 1 mM to 10 mM, secreted insulin rose from 40.3 +/- 0.8 to 56.3 +/- 3.2 mu IU/ml (STC-1-2), and from 10.8 +/- 0.8 to 23.6 +/- 2.3 mu IU/ml (STC-1-14). After STC-1-14 cells were implanted into diabetic nude mice, their blood glucose levels were reduced to normal. Body weight loss was also ameliorated. Our data suggested that genetically engineered K cells secrete active insulin in a glucose-regulated manner, and in vivo study showed that hyperglycemia could be reversed by implantation of the cells, suggesting that the use of genetically engineered K cells to express human insulin might provide a glucose-regulated approach to treat diabetic hyperglycemia. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | GENE DELIVERY ; ISLET TRANSPLANTATION ; INTESTINAL EPITHELIUM ; DIABETES-MELLITUS ; POLYPEPTIDE GENE ; IN-VIVO ; EXPRESSION ; MICE ; THERAPY ; PROINSULIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253501900008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1338]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, YQ,Yao, LQ,Shen, KT,et al. Genetically engineered K cells provide sufficient insulin to correct hyperglycemia in a nude murine model[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(2):149-157. |
| APA | Zhang, YQ.,Yao, LQ.,Shen, KT.,Xu, MD.,Zhou, PH.,...&Qin, XY.(2008).Genetically engineered K cells provide sufficient insulin to correct hyperglycemia in a nude murine model.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(2),149-157. |
| MLA | Zhang, YQ,et al."Genetically engineered K cells provide sufficient insulin to correct hyperglycemia in a nude murine model".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.2(2008):149-157. |
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