Cloning and isolation of a conus cysteine-rich protein homologous to Tex31 but without proteolytic activity
文献类型：期刊论文
| 作者 | Qian, J; Guo, ZY; Chi, CW |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:2页码:174-181 |
| 关键词 | cone snail cysteine-rich secretory protein protease protease inhibitor covalent modification |
| 通讯作者 | Guo, ZY (reprint author), Tongji Univ, Inst Prot Res, Shanghai 200092, Peoples R China.,zhan-yun.guo@mail.tongji.edu.cn ; zwqi@sibs.ac.cn |
| 英文摘要 | We cloned and isolated a cysteine-rich protein, designated Mr30, from Conus marmoreus. Mr30 belongs to the cysteinerich secretory protein family that is highly homologous to Tex31 previously obtained from Conus textile and reported as a protease responsible for processing of pro-conotoxins. Mr30, purified by a procedure similar to that of Tex31, indeed showed low proteolytic activity. However, further investigations revealed that the detected protease activity actually resulted from a trace amount of protease(s) contamination rather than from Mr30 itself. This finding led us to rethink the role of conus cysteine-rich secretory proteins: they were probably not responsible for the processing of pro-conotoxins as previously deduced, but their real biological functions remained to be clarified. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | MEDIATES GAMETE FUSION ; SECRETORY PROTEIN ; CRYSTAL-STRUCTURE ; CRISP FAMILY ; SNAKE-VENOM ; SPERM ; EXPRESSION ; CHANNEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253501900011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1339]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Qian, J,Guo, ZY,Chi, CW. Cloning and isolation of a conus cysteine-rich protein homologous to Tex31 but without proteolytic activity[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(2):174-181. |
| APA | Qian, J,Guo, ZY,&Chi, CW.(2008).Cloning and isolation of a conus cysteine-rich protein homologous to Tex31 but without proteolytic activity.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(2),174-181. |
| MLA | Qian, J,et al."Cloning and isolation of a conus cysteine-rich protein homologous to Tex31 but without proteolytic activity".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.2(2008):174-181. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。