Involvement of Sp1/Sp3 in the activation of the GATA-1 erythroid promoter in K562 cells
文献类型：期刊论文
| 作者 | Hou, CH; Huang, J; He, QY; Zhang, CN; Zhang, XJ; Qian, RL |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 18期号:2页码:302-310 |
| 关键词 | GATA-1 Sp1/Sp3 EKLF ChIP EMSA |
| 通讯作者 | Qian, RL (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Lab Mol Cell Biol, Shanghai 200031, Peoples R China.,xjzhang@sibs.ac.cn ; rlqian@sibs.ac.cn |
| 英文摘要 | GATA-1 is a hematopoietic transcription factor that is essential for the terminal maturation of proerythroblasts, megakaryocytic cells and mast cells. The erythroid-specific promoter of the human GATA-1 gene directs the high expression of a reporter gene in K562 cells. Multiple putative transcription factor binding sites were identified in the promoter from the -860 to the -1 base pair (bp). For a better understanding of the transcriptional control of human GATA-1 gene expression, we tested the transcriptional activity of a series of deletions from the 5' end of the 860-bp promoter. A region between -221 and -128 bp retains most of the transcriptional activity of the full-length promoter. Deletion of the CGCCC box at -195 bp reduced reporter gene activity to 60.4%. Further deletion of the CACCC box at -173 bp nearly abolished reporter gene expression, indicating that the CACCC box is more critical. In vitro experiments of electrophoretic mobility shifts and in vivo studies using chromatin immuno-precipitation (ChIP) assays show that the Sp1/Sp3 proteins bind the CACCC site in the nuclei of K562 cells. Coincidently, hyperacetylation of histones in the GATA-1 erythroid promoter was also shown by ChIP assay. Co-transfection of Sp1 expression plasmids and plasmids with a wild-type promoter showed enhanced reporter gene activity in a dose-dependent manner. The combined data demonstrate that Sp1/Sp3, but not EKLF, is involved in the activation of the GATA-1 erythroid promoter, and that histones H3 and H4 are highly acetylated in this promoter region for an actively transcribed GATA-1 gene in K562 cells in which EKLF is barely detectable. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | TRANSCRIPTION FACTOR GATA-1 ; KRUPPEL-LIKE FACTOR ; GENE-EXPRESSION ; IN-VIVO ; HEMATOPOIESIS ; LINEAGE ; EKLF ; SP1 ; UPSTREAM ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254024700011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1340]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hou, CH,Huang, J,He, QY,et al. Involvement of Sp1/Sp3 in the activation of the GATA-1 erythroid promoter in K562 cells[J]. CELL RESEARCH,2008,18(2):302-310. |
| APA | Hou, CH,Huang, J,He, QY,Zhang, CN,Zhang, XJ,&Qian, RL.(2008).Involvement of Sp1/Sp3 in the activation of the GATA-1 erythroid promoter in K562 cells.CELL RESEARCH,18(2),302-310. |
| MLA | Hou, CH,et al."Involvement of Sp1/Sp3 in the activation of the GATA-1 erythroid promoter in K562 cells".CELL RESEARCH 18.2(2008):302-310. |
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