Molecular cloning and characterization of two novel cellulase genes from the mollusc Ampullaria crossean
文献类型：期刊论文
| 作者 | Guo, R; Ding, M; Zhang, SL; Xu, GJ; Zhao, FK |
| 刊名 | JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY
 |
| 出版日期 | 2008 |
| 卷号 | 178期号:2页码:209-215 |
| 关键词 | cellulase endoglucanase EG27 Ampullaria crossean gene cloning |
| 通讯作者 | Zhao, FK (reprint author), Room 543,320 Yue Yang Rd, Shanghai 200031, Peoples R China.,fkzhao@sibs.ac.cn |
| 英文摘要 | Cellulase genes have been reported not only from fungi, bacteria and plant, but also from some invertebrate animals. Here, two cellulase (endo-beta-1,4-glucanase, EC 3.2.1.4) genes, eg27I and eg27II, were cloned from the freshwater snail Ampullaria crossean cDNA using degenerate primers. The nucleotide sequences of the two genes shared 94.5% identity. The open reading frames of both genes consisted of 588 bp, encoding 195 amino acids. Both EG27I and EG27II belong to the glycoside hydrolase family 45, and each lacks a carbohydrate-binding module. The presence of introns demonstrated a eukaryotic origin of the EG27 gene, and, in addition, successful cloning of EG27 cDNA supported endogenous production of EG27 cellulase by Ampullaria crossean. Investigation of the EG27 cDNA from A. crossean will provide further information on GHF45 cellulases. |
| 学科主题 | Physiology; Zoology |
| 类目[WOS] | Physiology ; Zoology |
| 关键词[WOS] | MULBERRY LONGICORN BEETLE ; ENDOGLUCANASE GENE ; ENZYMATIC-ACTIVITY ; APRIONA-GERMARI ; EXPRESSION ; PURIFICATION ; TERMITE ; ORIGIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000252483000008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1345]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, R,Ding, M,Zhang, SL,et al. Molecular cloning and characterization of two novel cellulase genes from the mollusc Ampullaria crossean[J]. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY,2008,178(2):209-215. |
| APA | Guo, R,Ding, M,Zhang, SL,Xu, GJ,&Zhao, FK.(2008).Molecular cloning and characterization of two novel cellulase genes from the mollusc Ampullaria crossean.JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY,178(2),209-215. |
| MLA | Guo, R,et al."Molecular cloning and characterization of two novel cellulase genes from the mollusc Ampullaria crossean".JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY 178.2(2008):209-215. |
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