aPKC inhibitors might be the sensitizer of chemotherapy and adoptive immunotherapy in the treatment of hASIPa - Overexpressed breast cancer
文献类型：期刊论文
| 作者 | Jin, YT; Ying, XX; Hu, YH; Zou, Q; Wang, HY; Xu, YH |
| 刊名 | ONCOLOGY RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 17期号:2页码:59-68 |
| 关键词 | human breast cancer hASIP aPKC inhibitor 5-FU DCIK/DCIK-P cells |
| 通讯作者 | Zou, Q (reprint author), Fudan Univ, Shanghai Med Sch, Huashan Hosp, Dept Surg, Shanghai 200040, Peoples R China.,zouqiang003@yahoo.com.cn |
| 英文摘要 | The atypical protein kinase C (aPKC) plays an important role in cell growth through the interaction with its substracts, including human ASIP (hASIP), which contains an aPKC phosphorylation site encoded by exon 17b of the gene. hASIP is expressed as numerous alternative splicing isoforms in the cells. Our results showed that hASIPa, an exon 17b-containing isoform of hASIP, is overexpressed in human breast cancer (HBC) MDA-MB-231, SK-BR-3, and MCF-7 cell lines and HBC specimens. The anticancer effects of 5-FU chemotherapy or adoptive immunotherapy and the synergic action of aPKC inhibitor against hASIPa-overexpressed HBC cells were tested. The results indicated that HBC MDA-MB-231 and SK-BR-3 cells were sensitive to 5-FU treatment in vitro. The combined treatment of aPKC inhibitor and 5-FU raised the anticancer activities against hASIPa-overexpressed HBC cells. The coculture of cytokine-induced killer (CIK) cells and autologous dendritic cells (DCs) with or without Her-2 peptide GP2 pulse created two new populations of effective immune-active T-cell populations called DC-modulated and cytokine-induced killer (DCIK) cells and peptide-DC-modulated and cytokine-induced killer (DCIK-P) cells. The DCIK cells showed cytotoxic activities on MDA-MB-231, SK-BR-3, and MCF-7 cells in MHC unrestricted manner. The DCIK-P cells possessed extra-enhanced cytotoxic activities against HLA-A2+/Her-2+ MDA-MB-231 cells in MHC restricted manner, but not for HLA-A2 +/-/Her-2+ SK-BR-3 cells and HLA-A2+Her-2 +/- MCF-7 cells. The data suggested specific cytotoxic T-lymphocyte (CTL) activity of DCIK-P cells on MDA-MB-231 cells. The combined treatment of aPKC inhibitor with DCIK/DCIK-P cells further raised the anticancer activities against hASIPa-overexpressed HBC cells. The results demonstrated that the hASIPa/aPKC signaling pathway functions as an important regulator in the growth of HBC cells and aPKC inhibitor treatment showed the synergic activities on 5-FU or DCIK/DCIK-P cells adoptive immunotherapy against hASIPa-overexpressed HBC cells. |
| 学科主题 | Oncology |
| 类目[WOS] | Oncology |
| 关键词[WOS] | INDUCED KILLER-CELLS ; KINASE-C-IOTA ; TRANSCRIPTION FACTOR ; DENDRITIC CELLS ; LUNG-CANCER ; PROTEIN ; POLARITY ; ISOFORMS ; SPECIFICITY ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000255969900002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1350]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jin, YT,Ying, XX,Hu, YH,et al. aPKC inhibitors might be the sensitizer of chemotherapy and adoptive immunotherapy in the treatment of hASIPa - Overexpressed breast cancer[J]. ONCOLOGY RESEARCH,2008,17(2):59-68. |
| APA | Jin, YT,Ying, XX,Hu, YH,Zou, Q,Wang, HY,&Xu, YH.(2008).aPKC inhibitors might be the sensitizer of chemotherapy and adoptive immunotherapy in the treatment of hASIPa - Overexpressed breast cancer.ONCOLOGY RESEARCH,17(2),59-68. |
| MLA | Jin, YT,et al."aPKC inhibitors might be the sensitizer of chemotherapy and adoptive immunotherapy in the treatment of hASIPa - Overexpressed breast cancer".ONCOLOGY RESEARCH 17.2(2008):59-68. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。