Transcriptome analysis of a cDNA library from adult human epididymis
文献类型：期刊论文
| 作者 | Li, JY; Wang, HY; Liu, J; Liu, Q; Zhang, JS; Wan, FC; Liu, FJ; Jin, SH; Zhang, YL |
| 刊名 | DNA RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 15期号:3页码:115-122 |
| 关键词 | human epididymis cDNA library transcriptomes for human epididymis sperm maturation |
| 通讯作者 | Li, JY (reprint author), Yu Huang Ding Hosp, Shandong Res Ctr Stem Cell Engn, Ctr Lab, Shandong 264000, Peoples R China.,sdscli@126.com ; ylzhang@sibs.ac.cn |
| 英文摘要 | Mammalian Gene Collection (MGC) verified over 9000 human full-ORF genes and FLJ Program reported 21 243 cDNAs of which 14 409 were unique ones and 5416 seemed to be protein-coding. The pity is that epididymis cDNA library was missing in their sequencing target list. Epididymis is a very important male accessory sex organ for sperm maturation and storage. Fully differentiated spermatozoa left from testis acquire their motility and capacity for fertilization via interactions with the epididymal epithelium duct lumen during passage through this convoluted duct. Here, we report that 20 000 clones from a healthy male epididymis cDNA library have been sequenced. The sequencing data provided 8234 known sequences and 650 unknown cDNA fragments. Hundred and six of 650 unknown cDNA clone inserts were randomly selected for fully sequencing. There were 2 5 unknown unique sequences and 19 released but unreported sequences came out. By northern blot analysis, four sequences randomly selected from the 19 released sequences with no known function showed positive mRNA signals in epididymis and testis. The signals for three of six from those unknown group showed as epididymis abundant in a region-specific manner but not in the testis and other tissues tested. All the sequencing data will be available on the website www.sdscli.com. |
| 学科主题 | Genetics & Heredity |
| 类目[WOS] | Genetics & Heredity |
| 关键词[WOS] | PROTEINS ; EXPRESSION ; GENOME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000258424600001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1355]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, JY,Wang, HY,Liu, J,et al. Transcriptome analysis of a cDNA library from adult human epididymis[J]. DNA RESEARCH,2008,15(3):115-122. |
| APA | Li, JY.,Wang, HY.,Liu, J.,Liu, Q.,Zhang, JS.,...&Zhang, YL.(2008).Transcriptome analysis of a cDNA library from adult human epididymis.DNA RESEARCH,15(3),115-122. |
| MLA | Li, JY,et al."Transcriptome analysis of a cDNA library from adult human epididymis".DNA RESEARCH 15.3(2008):115-122. |
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