Molecular characterization and immunohistochemical localization of a novel troponin C during silkworm development
文献类型：期刊论文
| 作者 | Chen, JQ; Chen, J; Gai, QJ; Lv, ZB; Wang, D; Nie, ZM; Wang, J; Wang, XD; Wu, XF; Zhang, YZ |
| 刊名 | CELL AND TISSUE RESEARCH
 |
| 出版日期 | 2008 |
| 卷号 | 331期号:3页码:725-738 |
| 关键词 | troponin C development expression pattern immunohistochemistry Bombyx mori (Insecta) |
| 通讯作者 | Zhang, YZ (reprint author), Zhejiang Sci Tech Univ, Inst Biochem, 2nd St,Xiasha Higher Educ Zone, Hangzhou 310018, Peoples R China.,yaozhou@chinagene.com |
| 英文摘要 | We have cloned and sequenced a novel Bombyx mori gene that encodes a protein having a high degree of homology with other known troponin C (TnC) proteins. The amino acid sequence, DX[DN]X[DSG]X(6)E, a highly conserved putative Ca(2+)-binding motif found in loops within the globular domains of previously identified TnC proteins, is also present in BmTnC. We have expressed and purified to homogeneity a His-tagged BmTnC fusion protein having a molecular weight of approximately 21.6 kDa. We have used this purified fusion protein to produce polyclonal antibodies against BmTnC for Western blot analyses. These analyses have revealed that BmTnC is expressed in the larval head, the Malpighian tubule, the epidermis, the testis, and the gut, as has been confirmed by immunohistochemistry. In addition, real-time reverse transcription/polymerase chain reaction has shown that BmTnC mRNA levels differ substantially among these tissues. Our findings indicate that BmTnC is selectively expressed in the muscular tissues of the silkworm, including portions of the head, the Malpighian tubule, the body wall, and the gut. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | MUSCLE-CONTRACTION ; SKELETAL-MUSCLE ; CALCIUM-BINDING ; EF-HAND ; PROTEINS ; EXPRESSION ; SENSITIZER ; DROSOPHILA ; RESOLUTION ; ISOFORMS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253128300013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1359]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, JQ,Chen, J,Gai, QJ,et al. Molecular characterization and immunohistochemical localization of a novel troponin C during silkworm development[J]. CELL AND TISSUE RESEARCH,2008,331(3):725-738. |
| APA | Chen, JQ.,Chen, J.,Gai, QJ.,Lv, ZB.,Wang, D.,...&Zhang, YZ.(2008).Molecular characterization and immunohistochemical localization of a novel troponin C during silkworm development.CELL AND TISSUE RESEARCH,331(3),725-738. |
| MLA | Chen, JQ,et al."Molecular characterization and immunohistochemical localization of a novel troponin C during silkworm development".CELL AND TISSUE RESEARCH 331.3(2008):725-738. |
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