Nudel binds Cdc42GAP to modulate Cdc42 activity at the leading edge of migrating cells
文献类型：期刊论文
| 作者 | Shen, YD; Li, N; Wu, S; Zhou, YH; Shan, YL; Zhang, QG; Ding, C; Yuan, Q; Zhao, FK; Zeng, R |
| 刊名 | DEVELOPMENTAL CELL
 |
| 出版日期 | 2008 |
| 卷号 | 14期号:3页码:342-353 |
| 通讯作者 | Zhu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn |
| 英文摘要 | Cdc42GAP promotes inactivation of Cdc42, a small GTPase whose activation at the leading edge by guanine nucleotide exchange factors is critical for cell migration. How Cdc42GAP is regulated to ensure proper levels of active Cdc42 is poorly understood. Here we show that Nudel, a cytoplasmic dynein regulator, competes with Cdc42 for binding Cdc42GAP. Consequently, Nudel can inhibit Cdc42GAP-mediated inactivation of Cdc42 in a dose-dependent manner. Both Nudel and Cdc42GAP exhibit leading-edge localization in migrating cells. The localization of Nudel requires its phosphorylation by Erk1/2. Depleting Nudel by RNAi or overexpression of a nonphosphorylatable mutant abolishes Cdc42 activation and cell migration. Our data thus uncover Nudel as a regulator of Cdc42 during cell migration. Nudel facilitates cell migration by sequestering Cdc42GAP at the leading edge to stabilize active Cdc42 in response to extracellular stimuli. Excess active Cdc42 may in turn control its own activity by recruiting Cdc42GAP from Nudel. |
| 学科主题 | Cell Biology; Developmental Biology |
| 类目[WOS] | Cell Biology ; Developmental Biology |
| 关键词[WOS] | CYTOPLASMIC DYNEIN ; MITOTIC SPINDLE ; LIS1 ; GTPASE ; RHO ; COMPLEX ; ASSOCIATION ; ACTIVATION ; DYNACTIN ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254115000009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1360]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Shen, YD,Li, N,Wu, S,et al. Nudel binds Cdc42GAP to modulate Cdc42 activity at the leading edge of migrating cells[J]. DEVELOPMENTAL CELL,2008,14(3):342-353. |
| APA | Shen, YD.,Li, N.,Wu, S.,Zhou, YH.,Shan, YL.,...&Zhu, XL.(2008).Nudel binds Cdc42GAP to modulate Cdc42 activity at the leading edge of migrating cells.DEVELOPMENTAL CELL,14(3),342-353. |
| MLA | Shen, YD,et al."Nudel binds Cdc42GAP to modulate Cdc42 activity at the leading edge of migrating cells".DEVELOPMENTAL CELL 14.3(2008):342-353. |
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