Identification of WSB1 gene as an important regulator in the development of zebrafish embryo during midblastula transition
文献类型：期刊论文
| 作者 | Lv, WJ; Zhang, YB; Wu, ZL; Chu, L; Koide, SS; Chen, YG; Yan, YC; Li, YP |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:6页码:478-488 |
| 关键词 | zebrafish microarray WSB1 embryo development midblastula transition |
| 通讯作者 | Yan, YC (reprint author), Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,ycyan@sibs.ac.cn ; yipingli@sibs.ac.cn |
| 英文摘要 | To uncover novel genes potentially involved in embryo development, especially at the midblastula transition (MBT) phase in the developing embryo of zebrafish, Affymetrix zebrafish GeneChip microarray analysis was carried out on the expression of 14,900 gene transcripts. The results of the analysis showed that 360 genes were clearly up-regulated and 119 genes were markedly down-regulated. Many of these genes were involved in transcription factor activity, nucleic acid binding, and cell growth. The present study showed that significant changes in transcript abundance occurred during the MBT phase. The expression of eight of these 479 genes was identified by reverse transcription-polymerase chain reaction analysis, confirming the microarray results. The WSB1 gene, found to be down-regulated by the microarray and reverse transcription-polymerase chain reaction analyses, was selected for further study. Sequence analysis of the WSB1 gene showed that it encodes a protein with 75% identity to the corresponding active human orthologs. In addition, WSB1 gene expression was detected at a higher level at 2 h post fertilization and at a lower level at 4 h post fertilization, consistent with the chip results. Overexpression of the WSB1 gene can result in the formation of abnormalities in embryos, as determined by fluorescence-activated cell sorting. The present study showed unequivocally that the occurrence of WSB1 expression is an important event during the MBT phase in the development of zebrafish embryos. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | EARLY XENOPUS-EMBRYOS ; EXPRESSION PATTERNS ; CDNA MICROARRAY ; ZYGOTIC GENE ; IN-VIVO ; PROLIFERATION ; EMBRYOGENESIS ; TRANSCRIPTION ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000256851700003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1387]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Lv, WJ,Zhang, YB,Wu, ZL,et al. Identification of WSB1 gene as an important regulator in the development of zebrafish embryo during midblastula transition[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(6):478-488. |
| APA | Lv, WJ.,Zhang, YB.,Wu, ZL.,Chu, L.,Koide, SS.,...&Li, YP.(2008).Identification of WSB1 gene as an important regulator in the development of zebrafish embryo during midblastula transition.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(6),478-488. |
| MLA | Lv, WJ,et al."Identification of WSB1 gene as an important regulator in the development of zebrafish embryo during midblastula transition".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.6(2008):478-488. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。