Quantitative analysis of cytoplasmic actin gene promoter and nuclear polyhedrosis virus immediate-early promoter activities in various tissues of silkworm Bombyx mori using recombinant Autographa californica nuclear polyhedrosis virus as vector
文献类型：期刊论文
| 作者 | Zhang, Y; Zhang, X; Xia, HZ; Xue, YG; Wang, JY; Tian, BZ; Wei, ZG; Lu, CD |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:6页码:533-538 |
| 关键词 | promoter activity A3 IE-1 silkworm tissue rAcNPV |
| 通讯作者 | Lu, CD (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,cdlu@sibs.ac.cn |
| 英文摘要 | Cassettes harboring luciferase reporter driven by Bombyx mori cytoplasmic actin gene promoter (A3) (671 bp) and B. mori nuclear polyhedrosis virus immediate-early promoter (IE-1) (580 bp) were transferred to the bacmid Ac Delta EGT to generate the recombinant Autographa californica nuclear polyhedrosis viruses, AcNPVA3Luc and AcNPVIELuc, respectively. Recombinant baculoviruses were injected into the hemocoele of newly ecdysed 5th instar larvae. The activities of the A3 and IE-1 promoters in various tissues were measured by luciferase activity assay and normalized by the copy number of recombinant virus. Results showed that the activity of the A3 promoter was approximately 10-fold higher than the IE-1 promoter. The promoter activities of A3 and IE-1 were highest in the silk gland, followed by fat body, middle gut, Malpighian tubule, and hemocyte. In silk gland, activity of the two promoters was highest in posterior silk gland, followed by middle and anterior silk glands. The difference in promoter activities reflects the growth speed of tissue in silkworm larvae. The activity of the A3 promoter remained unchanged and was not inhibited significantly by viral factors at least 3-4 d post injection of rAcNPV. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | EXPRESSION ; SYSTEM ; EGFP |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000256851700010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1389]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Y,Zhang, X,Xia, HZ,et al. Quantitative analysis of cytoplasmic actin gene promoter and nuclear polyhedrosis virus immediate-early promoter activities in various tissues of silkworm Bombyx mori using recombinant Autographa californica nuclear polyhedrosis virus as vector[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(6):533-538. |
| APA | Zhang, Y.,Zhang, X.,Xia, HZ.,Xue, YG.,Wang, JY.,...&Lu, CD.(2008).Quantitative analysis of cytoplasmic actin gene promoter and nuclear polyhedrosis virus immediate-early promoter activities in various tissues of silkworm Bombyx mori using recombinant Autographa californica nuclear polyhedrosis virus as vector.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(6),533-538. |
| MLA | Zhang, Y,et al."Quantitative analysis of cytoplasmic actin gene promoter and nuclear polyhedrosis virus immediate-early promoter activities in various tissues of silkworm Bombyx mori using recombinant Autographa californica nuclear polyhedrosis virus as vector".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.6(2008):533-538. |
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