Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis
文献类型：期刊论文
| 作者 | Yang, S; Liu, X; Yin, YQ; Fukuda, MN; Zhou, JW |
| 刊名 | FASEB JOURNAL
 |
| 出版日期 | 2008 |
| 卷号 | 22期号:6页码:1960-1972 |
| 关键词 | microtubules mitotic block |
| 通讯作者 | Zhou, JW (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Lab Mol Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,jwzhou@ion.ac.cn |
| 英文摘要 | Tastin was previously characterized as an accessory protein for cell adhesion that participates in early embryo implantation. Here, we report that tastin is also required for spindle assembly during mitosis. Tastin protein levels peaked in the G(2)/M phase and abruptly declined after cell division. Microscopy showed that tastin is primarily localized on the microtubules, centrosomes, and the mitotic spindle during the cell cycle. Tastin interacted with the dynein intermediate chain, p150(Glued), and gamma-tubulin in addition to Tctex-1 ( the light chain of dynein). Overexpression of tastin led to monopolar spindle formation, whereas loss of tastin expression caused profound mitotic block and preferentially induced multipolar spindles. These multipolar spindles were generated through a loss of cohesion in mitotic centrosomes; specifically, tastin depletion caused the fragmentation of pericentrosomal material and the splitting of the centrioles at the spindle poles. Tastin depletion induced centrosome abnormalities exclusively during mitosis and required both microtubule integrity and Eg5 activity. However, tastin depletion did not disrupt the organization of spindle poles, as revealed by localization of nuclear mitotic apparatus protein (NuMA) and the p150(Glued) component of dynactin. These data indicate that the major function of tastin during mitosis is to maintain the structural and dynamic features of centrosomes, thereby contributing to spindle bipolarity. |
| 学科主题 | Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biology ; Cell Biology |
| 关键词[WOS] | ENDOMETRIAL EPITHELIAL-CELLS ; MITOTIC SPINDLE ; CYTOPLASMIC DYNEIN ; LIGHT-CHAIN ; PROTEIN ; COMPLEX ; ORGANIZATION ; MECHANISMS ; TROPHININ ; INTERACTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000256352700035 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1393]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, S,Liu, X,Yin, YQ,et al. Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis[J]. FASEB JOURNAL,2008,22(6):1960-1972. |
| APA | Yang, S,Liu, X,Yin, YQ,Fukuda, MN,&Zhou, JW.(2008).Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis.FASEB JOURNAL,22(6),1960-1972. |
| MLA | Yang, S,et al."Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis".FASEB JOURNAL 22.6(2008):1960-1972. |
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